Winter Term 2021/2022
Because of the measures taken to mitigate the Covid-19 pandemic teaching in the winter term cannot be carried out as usual. In this Moodle course you can find an overview of changes for all courses in the biochemistry bachelor and master studies courses. This overview will be continuously supplemented and updated.
Lecture Biochemistry III
Prof. Dr. Michael Hollmann
Start of lecture
Wednesday, DD.10.2021
Time
Wednesdays 13:00 c.t. – 15:00
Location
Lecture hall NC 6/99
Exam 1
Wednesday, 02.02.2022, 14:00 – 16:00, HZO 40
Exam 2
Wednesday, 16.03.2022, 14:00 – 16:00, HZO 40
Register on eCampus for written exams.
Topics
- Structure of eukaryotic cells
- Chromosome structure
- Replication
- Recombination
- Transposable elements
- Reassociation of nucleic acids
- DNA cloning and gene technology (“applied hybridisations”)
- Transcription
- RNA polymerase I
- RNA polymerase III
- Ribozymes
- Regulation of gene expression in eukaryotes
- Translocation of proteins
- Cell cycle
- Immune system
Biochemical Practicals
Praktikum Biochemische Arbeitstechniken
Anmeldung
16.08.2021 bis 10.09.2021 über eCampus
Einführungsveranstaltung
Montag, 11.10.2021, 15.00 Uhr über ZOOM
Praktikumstermine
Über das gesamte Semester jeweils donnerstags ganztägig im Praktikumssaal NBCF 04/252
Weitere Informationen und das Skript zum Praktikum finden Sie in Moodle. Alle angemeldeten Teilnehmer werden automatisch in den Moodle-Kurs eingeschrieben.
Molekularbiologisches Praktikum
Anmeldung
28.06.2021 bis 11.07.2021 über eCampus
Einführungsveranstaltung
Donnerstag, 15.07.2021, 9.15 Uhr in der Vorlesung Molekulargenetische Methoden in der Biochemie über ZOOM
Praktikumstermine
Die vier Versuche finden an jeweils zwei Tagen im Praktikumssaal NBCF 04/252 statt: 13./14.09.2021, 15./16.09.2021, 20./21.09.2021, 23./24.09.2021, 27./28.09.2021
Weitere Informationen und das Skript zum Praktikum finden Sie in Moodle. Das Paßwort wird in der Einführungsveranstaltung bekanntgegeben.
Laborpraktikum Biochemie für Fortgeschrittene
Im Laborpraktikum stellen sich die biochemischen Arbeitsgruppen der Fakultät vor. Am Lehrstuhl für Biochemie I bieten Prof. Hollmann sechs und Dr. Tapken vier Termine an. Das Praktikum soll als Entscheidungsgrundlage für die Bewerbung um einen Platz für die Bachelorarbeit dienen, die in der Regel im folgenden Sommersemester angefertigt wird. Plätze für die Bachelorarbeit vergeben wir deshalb erst nach Ende des Laborpraktikums Ende Januar.
Im Rahmen des Laborpraktikums hält außerdem jeder Teilnehmer einen Seminarvortrag bei einem der Dozenten.
Anmeldung
27.09.2021, 12.00 Uhr bis 15.10.2021, 12.00 Uhr über eCampus
Einführungsveranstaltung
Dienstag, 26.10.2021, 11.00 Uhr über ZOOM
Vergabe der Seminarthemen
Ab Montag, 08.11.2021 bei dem jeweiligen Dozenten
Praktikumstermine
Jeweils dienstags: 30.11.2021, 07.12.2021, 14.12.2021, 11.01.2022, 18.01.2022, 25.01.2022
Weitere Informationen zum Praktikum, die Gruppeneinteilung und eine Liste der Praktikumsräume finden Sie in Moodle. Bis zum Anmeldeschluß ist die Einschreibung ohne Paßwort möglich.
Biochemisches Wahlpraktikum
Methoden der Zellkultur und konfokale Mikroskopie
Christina Klein-Schmidt und Dr. Daniel Tapken
Praktikumstermine
10./11.02.2022, 17./18.02.2022
Affinitätsreinigung und Western-Blot-Analyse von Glutamatrezeptoren aus Xenopus-laevis-Oozyten
Dr. Ralf Trippe
Praktikumstermine
Modular Advanced Practical
Heterologous Expression of Neurotransmitter Receptors in Frog Oocytes
In this course, the strategies and techniques necessary for studying structure-function relationships on recombinant neurotransmitter receptors and other ion channels are demonstrated using glutamate receptors as an example. Molecular biological, electrophysiological and protein biochemical techniques are utilized, which illustrates that a great variety of methods is required for solving modern questions in neurobiochemistry. This semester only the electrophysiology part will be offered.
The course is offered three times in the winter term 2021/2022.
15.11. – 26.11.2021 Dr. Daniel Tapken
06.12. – 17.12.2021 Amina Bećić
24.01. – 04.02.2022 Javeria Shaukat
The script for the modular advanced practical is available from Dr. Tapken approximately one week before the respective course starts.
Supervisors:
Dr. Daniel Tapken, Room NC 6/132, Tel. +49 234 32-24233
Jennifer Leifeld, Room NC 6/174, Tel. +49 234 32-28324
Amina Bećić, Room NC 6/131, Tel. +49 234 32-27496
Javeria Shaukat, Room NC 6/131, Tel. +49 234 32-27496
Programme:
- Introductory lecture Dr. Daniel Tapken
Molecular Biology Part (only covered theoretically this semester)
- Transformation of bacteria
- Inoculation of bacterial cultures
- Small-scale preparation of plasmid DNA
- Restriction analysis of plasmid DNA (restriction and verification by agarose gel electrophoresis)
- Mid-scale preparation of plasmid DNA
- Linearisation of plasmids by restriction digest
- Check of linearisation efficiency by agarose gel electrophoresis
- Purification of template DNA
- In vitro transcription of the template
- Quantification of the cRNA yield
- Formaldehyde/agarose gel electrophoresis to check the quality of the cRNA
Electrophysiology Part
- Surgical preparation of frog oocytes
- Collagenase treatment of oocytes to remove follicle cells
- Selection of oocytes
- Preparation of pipettes for injection and microelectrodes for electrophysiological measurements of oocytes
- Microinjection of cRNA into oocytes
- Electrophysiological measurements on oocytes using the two-electrode voltage clamp technique
Autoantibodies against glutamate receptors
The course is offered twice in the winter term 2020/2021.
06.12. – 17.12.2021 Dr. Ralf Trippe
24.01. – 04.02.2022 Dr. Ralf Trippe
Supervisor:
Dr. Ralf Trippe, Room NC 6/167, Tel. +49 234 32-28240
Summer Term 2021
Because of the measures taken to mitigate the Covid-19 pandemic teaching in the summer term cannot be carried out as usual. In this Moodle course you can find an overview of changes for all courses in the biochemistry bachelor and master studies courses. This overview will be continuously supplemented and updated.
Lectures
Biochemistry II
Start of lecture
Monday, 12.04.2021
Execution of the lecture during contact-free teaching
The lecture will be held online via Zoom. The dates, Zoom links and further information can be found in Moodle. All provided materials will be available until the end of the semester. To obtain the password, please send an e-mail to Daniel Tapken.
Execution of the lecture in case of resumption of normal teaching
Mondays 12:15 to 14:15 in lecture hall HNC 30
Exam 1
Friday, 13.08.2021, 10:00 to 12:00, Lecture halls HZO 30, HZO 80, HNC 30
Exam 2
Monday, 13.09.2021, 10:00 to 12:00, Lecture halls HNC 10, HNC 20, HNC 30
Deadline for registration to the exam is 3 working days before the respective exam.
Topics
- History of the discovery of nucleic acids, their structure and function
- Nucleotide metabolism
- Structure and properties of DNA
- DNA replication in prokaryotes
- DNA repair mechanisms
- Structure and properties of RNA, RNA classes
- Transcription in prokaryotes
- RNA processing
- Regulation of transcription in prokaryotes
- Genetic code, tRNA and aminoacyl-tRNA synthetases
- Structure of ribosomes
- Translation in prokaryotes
- Translation in eukaryotes
Molecular Genetic Methods in Biochemistry
Dr. Daniel Tapken and Prof. Dr. Raphael Stoll
Start of lecture
Tuesday, 20.04.2021
Execution of the lecture during contact-free teaching
The lecture will be held online via Zoom. The dates, Zoom links and further information can be found in Moodle. All provided materials will be available until the end of the semester. To obtain the password, please send an e-mail to Daniel Tapken.
Execution of the lecture in case of resumption of normal teaching
Tuesdays 08:15 to 10:15 in lecture hall NC 6/99,
from 29.06. also Mondays, 12:15 to 14:15 in lecture hall HNC 30
Exam 1
Wednesday, 04.08.2021, 14:00 to 16:00, Lecture halls HNC 20, HZO 40
Exam 2
Wednesday, 22.09.2021, 14:00 to 16:00, Lecture halls HNC 20, HIC
Deadline for registration to the exam is 3 working days before the respective exam.
Topics
- Restriction endonucleases
- Escherichia coli as host organism
- Selection with antibiotics
- Plasmids and BACs
- Lambda phages and cosmids
- Single-stranded phages and phagemids
- Ligation
- Preparation of nucleic acids
- Electrophoresis of nucleic acids
- Nucleic acid hybridisation
- Polymerase chain reaction (PCR) and its applications
- DNA sequencing
- Genome editing
- Protein expression in E. coli
- Protein expression in eukaryotic cells
Biochemistry IV
Prof. Dr. Michael Hollmann and Prof. Dr. Thomas Günther-Pomorski
Start of lecture
Friday, 16.04.2021
Execution of the lecture during contact-free teaching
The lecture will be held online via Moodle. Further information on the individual dates, topics and technical implementation can also be found there. Please register via e-mail to Julia Schäfer.
Execution of the lecture in case of resumption of normal teaching
Fridays 10:00 c.t. to 12:00 in lecture hall NC 6/99
Exam 1
Wednesday, 11.08.2021, 14:00 to 16:00, Lecture halls HZO 40, HZO 50
Exam 2
Wednesday, 29.09.2021, 14:00 to 16:00, Lecture halls HIB, HNC 10, HNC 20
Deadline for registration to the exam is 7 days before the respective exam.
Lecture „Structure, Function and Physiology of nicotinic Acetylcholine Receptors“
Dr. Christoph Methfessel
Start of lecture
Tuesday, 13.04.2021
Execution of the lecture during contact-free teaching
The lecture will be held online via Moodle. Further information on the individual dates, topics and technical implementation can also be found there.
Execution of the lecture in case of resumption of normal teaching
Tuesdays 16:00 c.t. to 18:00 in seminar room NC 6/174
Exam 1
Thursday, DD.MM.2020, 10:00 to 12:00, Lecture hall
Exam 2
Thursday, DD.MM.2020, 10:00 to 12:00, Lecture hall
Deadline for registration to the exam is 7 days before the respective exam.
Biochemical Practicals
Praktikum Molekularbiologische Arbeitstechniken
Registration
01.04 to 18.04.2021 via eCampus
Introductory meeting
08.06.2021, 11:00 via Zoom
Course dates
Between 14.06.2021 and 13.07.2021 in the practical course hall NBCF 04/252.
The exact course dates, the script and further information can be found in in Moodle. All registered students will automatically be enrolled in the Moodle course.
Biochemisches Praktikum für Chemiker
Registration
01.04 to 18.04.2021 via eCampus
Introductory meeting
to be announced
Course dates
Between 29.04.2021 and 08.07.2021 in the practical course hall NBCF 04/252.
The exact course dates, the script and further information can be found in in Moodle. All registered students will automatically be enrolled in the Moodle course.
Focal Point Programme Biochemistry of the Nervous System
Further information is available here.