Summer Term 2024

Lectures

Biochemistry II

Dr. Daniel Tapken

Start of lecture

Monday, 08.04.2024

Time

Mondays 9:15 to 11:15
Lecture 2 Tuesday 9:15 to 11:15
Timetable

Location

Lecture hall NC 6/99

Exercises

There will be exercices during the second half of the term via Moodle and possibly after the lecture on Mondays from 11:15 to 13:00 in lecture hall NC 6/99. Details will be announced during the lecture and are available in Moodle.

Topics
  • History of the discovery of nucleic acids, their structure and function
  • Nucleotide metabolism
  • Structure and properties of DNA
  • DNA replication in prokaryotes
  • DNA repair mechanisms
  • Structure and properties of RNA, RNA classes
  • Transcription in prokaryotes
  • RNA processing
  • Regulation of transcription in prokaryotes
  • Genetic code, tRNA and aminoacyl-tRNA synthetases
  • Structure of ribosomes
  • Translation in prokaryotes
  • Translation in eukaryotes

Further information and materials can be found in Moodle. Enrolment is possible without a password until 07.04.2024.

Exam 1

Friday, 09.08.2024, 10:00 to 12:00, Lecture hall HIC

Exam 2

Monday, 16.09.2024, 10:00 to 12:00, Lecture hall HZO 30

Deadline for registration to the exam is 3 working days before the respective exam.

 

Molecular Genetic Methods in Biochemistry

Dr. Daniel Tapken and Prof. Dr. Raphael Stoll

Start of lecture

Tuesday, 16.04.2024

Time

Tuesdays 09:15 to 11:15
Lecture 9 Monday 9:15 to 11:15
Timetable

Location

Lecture hall NC 6/99

Topics
  • Restriction endonucleases
  • Escherichia coli as host organism
  • Selection with antibiotics
  • Plasmids and BACs
  • Lambda phages and cosmids
  • Single-stranded phages and phagemids
  • Ligation
  • Preparation of nucleic acids
  • Electrophoresis of nucleic acids
  • Nucleic acid hybridisation
  • Polymerase chain reaction (PCR) and its applications
  • DNA sequencing
  • Genome editing
  • Protein expression in E. coli
  • Protein expression in eukaryotic cells

Further information and materials can be found in Moodle. Enrolment is possible without a password until 17.04.2022.

Exam 1

Wednesday, 31.07.2024, 14:00 to 16:00, Lecture halls HNC 10

Exam 2

Wednesday, 25.09.2024, 14:00 to 16:00, Lecture hall HIC

Deadline for registration to the exam is 3 working days before the respective exam.

 

Biochemistry IV

Prof. Dr. Michael Hollmann and Prof. Dr. Thomas Günther-Pomorski

Start of lecture

Friday, 14.04.2023

Time

Fridays 10:15 to 12:00

Location

Lecture hall NC 6/99 or online via Zoom

Further information on the individual dates, topics and technical implementation can be found in Moodle. Please register via the Moodle course Biochemistry IV - Registration (185820-SoSe23) until 31.03.2023. After the end of the registration period you will obtain the password for the regular Biochemistry IV Moodle course.

Exam 1

Wednesday, 02.08.2023, 14:00 to 16:00, Lecture hall HZO 20

Exam 2

Wednesday, 04.10.2023, 14:00 to 16:00, Lecture hall HZO 20

Deadline for registration to the exam is 7 days before the respective exam.

 

Lecture „Structure, Function and Physiology of nicotinic Acetylcholine Receptors“

Dr. Christoph Methfessel

Start of lecture

Tuesday, 09.04.2024

Time

Tuesdays 16:15 to 18:00

Location

The first lecture on 09.04.2024 will be given in presence in the seminar room NC 6/174, all further lectures online via Moodle. Further information on the individual dates, topics and technical implementation can also be found there. Please register via e-mail to Martina Borkowski

Exam 1

Thursday, DD.MM.2024, 10:00 to 12:00, Lecture hall

Exam 2

Thursday, DD.MM.2024, 10:00 to 12:00, Lecture hall

Deadline for registration to the exam is 7 days before the respective exam.

 

Biochemical Practicals

Praktikum Molekularbiologische Arbeitstechniken

Registration

18.03 to 07.04.2024 via eCampus

Introductory meeting

17.06.2024, 11:15 in NC 6/99

Course dates

Between 08.07. and 19.07.2024 in the practical course hall NBCF 04/252.

The exact course dates, the script and further information can be found in in Moodle. All registered students will automatically be enrolled in the Moodle course.

 

Biochemisches Praktikum für Chemiker

Registration

18.03. to 07.04.2024 via eCampus

Introductory meeting

25.04.2024, 13:00 in the seminar room NC 6/174

Course dates

Between 02.05. and 04.07.2024 in the practical course hall NBCF 04/252.

The exact course dates, the script and further information can be found in in Moodle. All registered students will automatically be enrolled in the Moodle course.

 

Focal Point Programme Biochemistry of the Nervous System

Further information is available here.

 

Further Information

 

material

 

Winter Term 2023/2024

Important note: The lecture Bioinformatics in the Master Studies Course Biochemistry has been renamed. Please register for the course „Bioinformatics for Molecular and Cellular Structures“ (190702).

Biochemical Practicals

Praktikum Biochemische Arbeitstechniken

Anmeldung

25.09.2023 bis 15.10.2023 über eCampus

Einführungsveranstaltung

Donnerstag, 09.11.2023, 12.15 Uhr, NC 6/99

Praktikumstermine

Vom 16.11.2023 bis zum 25.01.2023 jeweils donnerstags ganztägig im Praktikumssaal NBCF 04/252

Wei­te­re In­for­ma­tio­nen und das Skript zum Prak­ti­kum fin­den Sie in Moodle. Al­le an­ge­mel­de­ten Teil­neh­mer wer­den au­to­ma­tisch in den Moodle-Kurs ein­ge­schrie­ben.

 

Molekularbiologisches Praktikum

Anmeldung

08.06.2023 bis 19.06.2023 über eCampus

Einführungsveranstaltung

Dienstag, 20.06.2023, 9.15 Uhr in der Vorlesung Molekulargenetische Methoden in der Biochemie

Praktikumstermine

Die vier Versuche finden an jeweils zwei Tagen im Praktikumssaal NBCF 04/252 statt: 19./20.09.2023, 21./22.09.2023, 25./26.09.2023, 28./29.09.2023

Wei­te­re In­for­ma­tio­nen und das Skript zum Prak­ti­kum fin­den Sie in Moodle. Das Paß­wort wird in der Ein­füh­rungs­ver­an­stal­tung be­kannt­ge­ge­ben.

 

Laborpraktikum Biochemie für Fortgeschrittene

Im La­bor­prak­ti­kum stel­len sich die bio­che­mi­schen Ar­beits­grup­pen der Fa­kul­tät vor. Am Lehr­stuhl für Bio­che­mie I bie­tet Dr. Tap­ken vier Ter­mi­ne an. Das Prak­ti­kum soll als Ent­schei­dungs­grund­la­ge für die Be­wer­bung um ei­nen Platz für die Ba­che­lor­ar­beit die­nen, die in der Re­gel im fol­gen­den Som­mer­se­me­ster an­ge­fer­tigt wird. Plät­ze für die Ba­che­lor­ar­beit ver­ge­ben wir des­halb erst nach En­de des La­bor­prak­ti­kums En­de Januar.

Im Rah­men des La­bor­prak­ti­kums hält au­ßer­dem je­der Teil­neh­mer ei­nen Se­mi­nar­vor­trag bei ei­nem der Do­zen­ten.

Anmeldung

02.10.2023, 12.00 Uhr bis 20.10.2023, 12.00 Uhr über eCampus

Einführungsveranstaltung

Dienstag, 24.10.2023, ND 03/99

Vergabe der Seminarthemen

Ab Montag, 30.10.2023 bei dem jeweiligen Dozenten

Praktikumstermine

Jeweils dienstags: 28.11.2023, 05.12.2023, 12.12.2023, 09.01.2023, 16.01.2023, 23.01.2023

Wei­te­re In­for­ma­tio­nen zum Prak­ti­kum, die Grup­pen­ein­tei­lung und ei­ne Li­ste der Prak­ti­kums­räu­me fin­den Sie in Moodle. Bis zum An­mel­de­schluß ist die Ein­schrei­bung ohne Paß­wort mög­lich.

 

Biochemisches Wahlpraktikum

Methoden der Zellkultur und konfokale Mikroskopie

Christina Klein-Schmidt und Dr. Daniel Tapken

Praktikumstermine

 

Affinitätsreinigung und Western-Blot-Analyse von Glutamatrezeptoren aus Xenopus-laevis-Oozyten

Dr. Ralf Trippe

Praktikumstermine

 

 

Modular Advanced Practical

Heterologous Expression of Neurotransmitter Receptors in Frog Oocytes

In this course, the strategies and techniques necessary for studying structure-function relationships on recombinant neurotransmitter receptors and other ion channels are demonstrated using glutamate receptors as an example. Molecular biological, electrophysiological and protein biochemical techniques are utilized, which illustrates that a great variety of methods is required for solving modern questions in neurobiochemistry. This semester only the electrophysiology part will be offered.

The course is offered once in the winter term 2023/2024.

23.10. – 03.11.2023 Dr. Daniel Tapken

The script for the modular advanced practical is available from Dr. Tapken approximately one week before the respective course starts.

Supervisor:

Dr. Daniel Tapken, Room NC 6/132, Tel. +49 234 32-24233

Programme:

  • Introductory lecture Dr. Daniel Tapken
Molecular Biology Part (only covered theoretically this semester)
  • Transformation of bacteria
  • Inoculation of bacterial cultures
  • Small-scale preparation of plasmid DNA
  • Restriction analysis of plasmid DNA (restriction and verification by agarose gel electrophoresis)
  • Mid-scale preparation of plasmid DNA
  • Linearisation of plasmids by restriction digest
  • Check of linearisation efficiency by agarose gel electrophoresis
  • Purification of template DNA
  • In vitro transcription of the template
  • Quantification of the cRNA yield
  • Formaldehyde/agarose gel electrophoresis to check the quality of the cRNA
Electrophysiology Part
  • Surgical preparation of frog oocytes
  • Collagenase treatment of oocytes to remove follicle cells
  • Selection of oocytes
  • Preparation of pipettes for injection and microelectrodes for electrophysiological measurements of oocytes
  • Microinjection of cRNA into oocytes
  • Electrophysiological measurements on oocytes using the two-electrode voltage clamp technique

Autoantibodies against glutamate receptors

The course is offered once in the winter term 2023/2024.

04.12. – 15.12.2023

Supervisor:

Dr. Ralf Trippe, Room NC 6/167, Tel. +49 234 32-28240