Winter Term 2020/2021

Because of the measures taken to mitigate the Covid-19 pandemic teaching in the winter term cannot be carried out as usual. In this Moodle course you can find an overview of changes for all courses in the biochemistry bachelor and master studies courses. This overview will be continuously supplemented and updated.

Lecture Biochemistry III

Prof. Dr. Michael Hollmann

Start of lecture

Wednesday, 28.10.2020

Time

Wednesdays 13:00 c.t. – 15:00

Location

Lecture hall NC 6/99

Exam 1

Wednesday, 03.03.2021, 14:00 – 16:00, HNC 10

Exam 2

Wednesday, 24.03.2021, 14:00 – 16:00, HNC 10

Register on eCampus for written exams.

Topics
  • Structure of eukaryotic cells
  • Chromosome structure
  • Replication
  • Recombination
  • Transposable elements
  • Reassociation of nucleic acids
  • DNA cloning and gene technology (“applied hybridisations”)
  • Transcription
  • RNA polymerase I
  • RNA polymerase III
  • Ribozymes
  • Regulation of gene expression in eukaryotes
  • Translocation of proteins
  • Cell cycle
  • Immune system

Biochemical Practicals

Praktikum Biochemische Arbeitstechniken

Anmeldung

06.10.2020, 12.00 Uhr bis 22.10.2020, 12.00 Uhr über eCampus

Einführungsveranstaltung

Montag, 26.10.2020, 10.00 Uhr über ZOOM

Praktikumstermine

Über das gesamte Semester jeweils donnerstags ganztägig im Praktikumssaal NBCF 04/252

Wei­te­re In­for­ma­tio­nen und das Skript zum Prak­ti­kum fin­den Sie in Moodle. Bis zum An­mel­de­schluß ist die Ein­schrei­bung ohne Paß­wort mög­lich.

 

Laborpraktikum Biochemie für Fortgeschrittene

Im La­bor­prak­ti­kum stel­len sich die bio­che­mi­schen Ar­beits­grup­pen der Fa­kul­tät vor. Am Lehr­stuhl für Bio­che­mie I bie­ten Prof. Holl­mann sechs und Dr. Tap­ken vier Ter­mi­ne an. Das Prak­ti­kum soll als Ent­schei­dungs­grund­la­ge für die Be­wer­bung um ei­nen Platz für die Ba­che­lor­ar­beit die­nen, die in der Re­gel im fol­gen­den Som­mer­se­me­ster an­ge­fer­tigt wird. Plät­ze für die Ba­che­lor­ar­beit ver­ge­ben wir des­halb erst nach En­de des La­bor­prak­ti­kums En­de Januar.

Im Rah­men des La­bor­prak­ti­kums hält au­ßer­dem je­der Teil­neh­mer ei­nen Se­mi­nar­vor­trag bei ei­nem der Do­zen­ten.

Anmeldung

06.10.2020, 12.00 Uhr bis 22.10.2020, 12.00 Uhr über eCampus

Einführungsveranstaltung

Dienstag, 27.10.2020, 10.00 Uhr über ZOOM

Vergabe der Seminarthemen

Ab Montag, 09.11.2020 bei dem jeweiligen Dozenten

Praktikumstermine

Jeweils dienstags: 01.12.2020, 08.12.2020, 15.12.2020, 12.01.2021, 19.01.2021, 26.01.2021

Wei­te­re In­for­ma­tio­nen zum Prak­ti­kum, die Grup­pen­ein­tei­lung und ei­ne Li­ste der Prak­ti­kums­räu­me fin­den Sie in Moodle. Bis zum An­mel­de­schluß ist die Ein­schrei­bung ohne Paß­wort mög­lich.

 

Biochemisches Wahlpraktikum

Methoden der Zellkultur und konfokale Mikroskopie

Christina Klein-Schmidt und Dr. Daniel Tapken

Praktikumstermine

25./26.02.2021, 04./05.03.2021

Affinitätsreinigung und Western-Blot-Analyse von Glutamatrezeptoren aus Xenopus-laevis-Oozyten

Dr. Ralf Trippe

Praktikumstermine

 

 

Modular Advanced Practical

Heterologous Expression of Neurotransmitter Receptors in Frog Oocytes

In this course, the strategies and techniques necessary for studying structure-function relationships on recombinant neurotransmitter receptors and other ion channels are demonstrated using glutamate receptors as an example. Molecular biological, electrophysiological and protein biochemical techniques are utilized, which illustrates that a great variety of methods is required for solving modern questions in neurobiochemistry. This semester only the electrophysiology part will be offered.

The course is offered five times in the winter term 2020/2021.

16.11. – 26.11.2020 Dr. Daniel Tapken

07.12. – 17.12.2020 Jennifer Leifeld

11.01. – 21.01.2021 Amina Bećić

01.02. – 11.02.2021 Javeria Shaukat

22.02. – 04.03.2021 Dr. Daniel Tapken

The script for the modular advanced practical is available from Dr. Tapken approximately one week before the respective course starts.

Supervisors:

Dr. Daniel Tapken, Room NC 6/132, Tel. +49 234 32-24233
Jennifer Leifeld, Room NC 6/174, Tel. +49 234 32-28324
Amina Bećić, Raum NC 6/131, Tel. +49 234 32-27496
Javeria Shaukat, Raum NC 6/131, Tel. +49 234 32-27496

Programme:

  • Introductory lecture Dr. Daniel Tapken
Molecular Biology Part (only covered theoretically this semester)
  • Transformation of bacteria
  • Inoculation of bacterial cultures
  • Small-scale preparation of plasmid DNA
  • Restriction analysis of plasmid DNA (restriction and verification by agarose gel electrophoresis)
  • Mid-scale preparation of plasmid DNA
  • Linearisation of plasmids by restriction digest
  • Check of linearisation efficiency by agarose gel electrophoresis
  • Purification of template DNA
  • In vitro transcription of the template
  • Quantification of the cRNA yield
  • Formaldehyde/agarose gel electrophoresis to check the quality of the cRNA
Electrophysiology Part
  • Surgical preparation of frog oocytes
  • Collagenase treatment of oocytes to remove follicle cells
  • Selection of oocytes
  • Preparation of pipettes for injection and microelectrodes for electrophysiological measurements of oocytes
  • Microinjection of cRNA into oocytes
  • Electrophysiological measurements on oocytes using the two-electrode voltage clamp technique

Autoantibodies against glutamate receptors

The course is offered twice in the winter term 2020/2021.

07.12. – 17.12.2020 Dr. Ralf Trippe

01.02. – 11.02.2021 Dr. Ralf Trippe

Supervisor:

Dr. Ralf Trippe, Room NC 6/167, Tel. +49 234 32-28240

 

Summer Term 2020

Because of the measures taken to mitigate the Covid-19 pandemic teaching in the summer term cannot be carried out as usual. In this Moodle course you can find an overview of changes for all courses in the biochemistry bachelor and master studies courses. This overview will be continuously supplemented and updated.

Lectures

Biochemistry II

Dr. Daniel Tapken

Start of lecture

Monday, 06.04.2020

Execution of the lecture during contact-free teaching

The lecture will be held online via Moodle. Further information on the individual dates, topics and technical implementation can also be found there. All provided materials will be available until the end of the semester. To obtain the password, please send an e-mail to Daniel Tapken.

Execution of the lecture in case of resumption of normal teaching

Mondays 12:15 to 14:15 in lecture hall HNC 30

Exam 1

Friday, 07.08.2020, 10:00 to 12:00, Lecture hall HZO 30

Exam 2

Monday, 14.09.2020, 10:00 to 12:00, Lecture hall HGA 10

Deadline for registration to the exam is 3 working days before the respective exam.

Topics
  • History of the discovery of nucleic acids, their structure and function
  • Nucleotide metabolism
  • Structure and properties of DNA
  • DNA replication in prokaryotes
  • DNA repair mechanisms
  • Structure and properties of RNA, RNA classes
  • Transcription in prokaryotes
  • RNA processing
  • Regulation of transcription in prokaryotes
  • Genetic code, tRNA and aminoacyl-tRNA synthetases
  • Structure of ribosomes
  • Translation in prokaryotes
  • Translation in eukaryotes

 

Molecular Genetic Methods in Biochemistry

Dr. Daniel Tapken and Prof. Dr. Raphael Stoll

Start of lecture

Tuesday, 21.04.2020

Execution of the lecture during contact-free teaching

The lecture will be held online via Moodle. Further information on the individual dates, topics and technical implementation can also be found there. All provided materials will be available until the end of the semester. To obtain the password, please send an e-mail to Daniel Tapken.

Execution of the lecture in case of resumption of normal teaching

Tuesdays 08:15 to 10:15 in lecture hall NC 6/99,
from 29.06. also Mondays, 12:15 to 14:15 in lecture hall HNC 30

Exam 1

Wednesday, 29.07.2020, 14:00 to 16:00, Lecture halls HZO 50 and HGB 30

Exam 2

Wednesday, 23.09.2020, 14:00 to 16:00, Lecture hall HZO 40

Deadline for registration to the exam is 3 working days before the respective exam.

Topics
  • Restriction endonucleases
  • Escherichia coli as host organism
  • Selection with antibiotics
  • Plasmids and BACs
  • Lambda phages and cosmids
  • Single-stranded phages and phagemids
  • Ligation
  • Preparation of nucleic acids
  • Electrophoresis of nucleic acids
  • Nucleic acid hybridisation
  • Polymerase chain reaction (PCR) and its applications
  • DNA sequencing
  • Genome editing
  • Protein expression in E. coli
  • Protein expression in eukaryotic cells

 

Biochemistry IV

Prof. Dr. Michael Hollmann and Prof. Dr. Thomas Günther-Pomorski

Start of lecture

Friday, 24.04.2020

Execution of the lecture during contact-free teaching

The lecture will be held online via Moodle. Further information on the individual dates, topics and technical implementation can also be found there. Please register via e-mail to Julia Schäfer.

Execution of the lecture in case of resumption of normal teaching

Fridays 10:00 c.t. to 12:00 in lecture hall NC 6/99

Exam 1

Wednesday, 05.08.2020, 14:00 to 16:00, Lecture hall HIB

Exam 2

Wednesday, 30.09.2020, 14:00 to 16:00, Lecture hall HZO 40

Deadline for registration to the exam is 7 days before the respective exam.

 

Lecture „Structure, Function and Physiology of nicotinic Acetylcholine Receptors“

Dr. Christoph Methfessel

Start of lecture

Tuesday, DD.MM.2020

Execution of the lecture during contact-free teaching

The lecture will be held online via Moodle. Further information on the individual dates, topics and technical implementation can also be found there.

Execution of the lecture in case of resumption of normal teaching

Tuesdays 16:00 c.t. to 18:00 in seminar room NC 6/174

Exam 1

Thursday, DD.MM.2020, 10:00 to 12:00, Lecture hall HXX

Exam 2

Thursday, DD.MM.2020, 10:00 to 12:00, Lecture hall HYY

Deadline for registration to the exam is 7 days before the respective exam.

 

Biochemical Practicals

The biochemical practicals will presumably not be conducted as planned and specified below. As soon as more information is available, it will be announced here and in this Moodle course.

Praktikum Molekularbiologische Arbeitstechniken

Registration

until 12.06.2020 via E-mail to Prof. Hollmann

Introductory meeting

to be announced

Course dates

Between 29.06.2020 and 21.07.2020 in the practical course hall NBCF 04/252. The exact dates will be announced later.

Further information and the script are available in Moodle. The password will be announced during the introductory meeting.

 

Biochemisches Praktikum für Chemiker

Registration

until 12.06.2020 via E-mail to Prof. Hollmann

Introductory meeting

to be announced

Course dates

Between 29.06.2020 and 21.07.2020 in the practical course hall NBCF 04/252. The exact dates will be announced later.

Further information and the script are available in Moodle. The password will be announced during the introductory meeting.

 

Focal Point Programme Biochemistry of the Nervous System

Further information is available here.

Further Information

 

material