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Terahertz (THz)-Spektroskopie

THz-Tanz des Wassers

In flüssiger Phase sind Wassermoleküle über Wasserstoff-brücken miteinander verbunden. Die Fluktuationen im Wassernetzwerk erfolgen auf der sub-ps- und ps-Zeitskala. THz-Absorptionsmessungen (1 THz = 1012 Hz = 1 ps-1) "sehen" diese dynamischen Umorientierungen der Dipolmomente und damit die Änderungen in den kollektiven Bewegungen des Wassernetzwerks durch gelöste Stoffe.



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Zu unseren Publikationen

THz-Spektroskopie der Solvation von Biomolekülen

Der Einfluss des Wassers auf die Dynamik von Biomolekülen ist ein wichtiger Forschungsgegenstand. THz-Spektroskopie ermöglicht die Messung kleinster Änderungen der Wassernetzwerkdynamik an der Biomolekül-Wasser-Grenzfläche.

Correlation with the Hofmeister series: This is how ionos and water interact microscopically
Lupe
Correlation with the Hofmeister series: This is how ionos and water interact microscopically
High pressure THz spectroskopy studies biological processes under deep sea conditions
Lupe
High pressure THz spectroskopy studies biological processes under deep sea conditions
THz calorimetry: THz spectra of solvated alcohol chains correlates with changes in heat capacity, entropy and free energy
Lupe
THz calorimetry: THz spectra of solvated alcohol chains correlates with changes in heat capacity, entropy and free energy

Kinetic THz absorption (KITA)

KITA has been developed in our group as a tool to probe changes in solvation dynamics upon biological function, e.g. protein folding, enzyme activity, water dynamics, calorimetry. With the help of the stopped flow and temperature jump technique, real-time changes in the protein-water network were detected during protein folding using terahertz absorption spectroscopy.


Relevanteste Publikationen zu diesem Thema
  • F.N. Brünig, M. Rammler, E.M. Adams, M. Havenith, R.R. Netz, Spectral signatures of excess-proton waiting and transfer-path dynamics in aqueous hydrochloric acid solutions, Nat. Commun., accepted (2022)
  • E. Adams, S. Pezzotti, J. Ahlers, M. Rüttermann, M. Levin, A. Goldenzweig, Y. Peleg, S. Fleischmann, I. Sagi, M. Havenith, Local mutations can serve as a game changer for global protein solvent interaction, JACS Au 1, 1076–1085 (2021)
  • S.R. Alfarano, S. Pezzotti, C.J. Stein, Z. Lin, F. Sebastiani, S. Funke, C. Hoberg, I. Kolling, C.Y. Ma, K. Mauelshagen, T. Ockelmann, G. Schwaab, L. Fu, J.-B. Brubach, P. Roy, M. Head-Gordon, K. Tschulik, M.-P. Gaigeot, M. Havenith, Stripping away ion hydration shells in electrical double layer formation: Water networks matter, PNAS 118, e2108568118-1–8 (2021)
  • V. Conti Nibali, S. Pezzotti, F. Sebastiani, D. Galimberti, G. Schwaab, M. Heyden, M.-P. Gaigeot, M. Havenith, Wrapping up hydrophobic hydration: Locality matters, J. Phys. Chem. Lett. 11, 4809–4816 (2020)
  • G. Schwaab, F. Sebastiani, M. Havenith, Ion hydration and ion pairing as probed by THz spectroscopy, Angew. Chem. Int. Ed. 58, 3000–3013 (2019)
  • F. Böhm, G. Schwaab, M. Havenith, Mapping hydration water around alcohol chains by THz calorimetry, Angew. Chem. Int. Ed. 56, 9981–9985 (2017)
  • S. Funkner, G. Niehues, D.A. Schmidt, M. Heyden, G. Schwaab, K.M. Callahan, D.J. Tobias, M. Havenith, Watching the low frequency motions in aqueous salt solutions: The terahertz vibrational signatures of hydrated ions, J. Am. Chem. Soc. 134, 1030–1035 (2012).
  • M. Grossman, B. Born, M. Heyden, D. Tworowski, G.B. Fields, I. Sagi, M. Havenith, Correlated structural kinetics and retarded solvent dynamics at the metalloprotease active site, Nat. Struct. Mol. Biol. 18, 1102–1108 (2011)
  • S.J. Kim, B. Born, M. Havenith, M. Gruebele, Real-time detection of protein-water dynamics upon protein folding by terahertz absorption Spectroscopy, Angew. Chem. Int. Ed. 47, 6486–6489 (2008)
  • S. Ebbinghaus, S.J. Kim, M. Heyden, X. Yu, U. Heugen, M. Gruebele, D.M. Leitner, M. Havenith, An extended dynamical solvation shell around proteins, PNAS 104, 20749–20752 (2007)