Analysis of DNA pools using second generation sequencing
Project code: SCHL 429/3-2
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We will study the genetic differentiation of B. didyma using Pool-Seq. Using the PoPoolation2 software we will determine basic population differentiation parameters, such as FST and Fisher's exact test. Furthermore, we will compare levels of polymorphism (pi, theta, number of alleles) for each of the populations studied. Using an FST based outlier approach we will identify genomic regions that carry putative functionally diverged loci. Finally, we will test if functionally diverged loci show a clinal pattern reflecting the differences in water availability.
Publications related to the project
- None at present
