3. Programmable chemical DNA ligation via disulphide bonds

Establish electric field controlled self-replication of SCP-DNAs in the absence of enzymes, via local concentration monitoring by changing the control voltages of the corresponding microelectrodes. Non-enzymatic replication of DNA (parabolic kinetics) is strongly sensitive to concentration effects, which have proven to be controllable via electric fields. Reversible cyclic modulation of pH by local electrolysis in flowing systems will also be employed to couple chemical replication with electronic signals.