Department of Cell Morphology and Molecular Neurobiology
Comparative analysis of the function of LRP1 in astroglial sub-lineages of the adult CNS in vivo
Astrocytes are glia cells of the central nervous system, which have contact to neurons, oligodendrocytes and blood vessels. Although astrocytes have diverse functions, like maintaining the pH and ion homeostasis, secretion of energy substrates and regulation of synaptic transmission. Alterations in the morphology or function of astrocytes are known as reactive gliosis, a reliable marker of injured and diseased tissue. The aim of this project is analysis of alteration in astrocytic sub-populations during development.
Short academic CV:
- 2010-2016: Studies of Biology at the Ruhr-University Bochum
- 2014: Bachelor of Science “Conditional LRP1-knock-out in spinal cord cells in vitro and the influence of the Erk 1/2/MAPK- and Akt/PI3K-signaling pathway on the differentiation of neural stem cells”
- 2016: Master of Science “Migration of LRP1-deficient neural stem cells in the developing brain”
- Since 2016: Scientific research assistant at the Department of Cell Morphology and Molecular Neurobiology
Applied methods
Cell biology:
- Cultivation of cortical astrocytes
- Immunocytochemistry
- Immunohistochemistry
- Microscopy (Axiophot, Axiovert, Laser-Scanning-Microscope))
Molecular biology:
- Isolation of DNA and RNA
- cDNA synthesis
- PCR
Protein biochemistry:
- Protein isolation
- SDS page
- Western Blot
