Proteomic studies
Stuhler K, Koper K, Pfeiffer K, Tagariello A, Souquet M, Schwarte-Waldhoff I, Hahn SA, Schmiegel W, Meyer HE. 
Differential proteome analysis of colon carcinoma cell line SW480 after reconstitution of the tumour suppressor Smad4. Anal Bioanal Chem. 2006 Nov;386(6):1603-12. Epub 2006 Oct 17. PMID: 17043799
Sitek B, Luttges J, Marcus K, Kloppel G, Schmiegel W, Meyer HE, Hahn SA, Stuhler K.
Application of fluorescence difference gel electrophoresis saturation labelling for the analysis of microdissected precursor lesions of pancreatic ductal adenocarcinoma. Proteomics. 2005 Jul;5(10):2665-79. PMID: 15924292
THE PROJECTS
Supported by the German Cancer Foundation (Deutsche Krebshilfe) and the
Federal Ministry of Education and Research (BMBF)
In collaboration with the Medical Proteome Center (MPC) state of the art proteomic technologies are applied to detect differentially expressed proteins from primary tumor cells and/or cell line model systems. Importantly, the MPC succeeded in developing a procedure including fluorescence dye saturation labeling in combination with two-dimensional gel electrophoresis for the analysis of proteins extracted from 1000 microdissected cells with a high resolution of up to 2500 protein spots (Sitek et al. ). This technology is currently been used to detect protein expression differences among microdissected PanIN-lesions and normal ductal cells.