DPC4-target genes
Stuhler K, Koper K, Pfeiffer K, Tagariello A, Souquet M, Schwarte-Waldhoff I, Hahn SA, Schmiegel W, Meyer HE.
Differential proteome analysis of colon carcinoma cell line SW480 after reconstitution of the tumour suppressor Smad4. Anal Bioanal Chem. 2006 Nov;386(6):1603-12. Epub 2006 Oct 17. PMID: 17043799
Volmer MW, Radacz Y, Hahn SA, Klein-Scory S, Stuhler K, Zapatka M, Schmiegel W, Meyer HE, Schwarte-Waldhoff I.
Tumor suppressor Smad4 mediates downregulation of the anti-adhesive invasion-promoting matricellular protein SPARC: Landscaping activity of Smad4 as revealed by a "secretome" analysis. Proteomics. 2004 May;4(5):1324-34. PMID: 15188399
Schwarte-Waldhoff I, Volpert OV, Bouck NP, Sipos B, Hahn SA, Klein-Scory S, Luttges J, Kloppel G,
Graeven U, Eilert-Micus C, Hintelmann A, Schmiegel W.
Smad4/DPC4-mediated tumor suppression through suppression of angiogenesis. Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9624-9. PMID: 10944227
Schwarte-Waldhoff I, Klein S, Blass-Kampmann S, Hintelmann A, Eilert C, Dreschers S, Kalthoff H,
Hahn SA, Schmiegel W.
DPC4/SMAD4 mediated tumor suppression of colon carcinoma cells is associated with reduced urokinase expression. Oncogene. 1999 May 20;18(20):3152-8. PMID: 10340387
INTRODUCTION
Deleted in Pancreatic Carcinoma Locus 4 (DPC4), belongs to the class of tumor suppressor genes. It was identified within chromosome band 18q21.1 which is frequently deleted in pancreatic carcinoma (Hahn et al. Science, 1996 ). DPC4 is a component of the transcription complex that mediates cell surface signals to the nucleus which are initiated by transforming growth factor β (TGFβ)-related growth and differentiation factors (Fig. 1).
Synonyms for DPC4
SMA- and MAD-related Protein 4; SMAD4
Deleted in pancreatic carcinoma locus 4; DPC4
Mother against decapentaplegic, drosophila, homolog of, 4; MADH4
Fig 1: Schematic view of the TGFbeta-Smad-Signalling pathway.
PROJECT DESCRIPTION
Together with I. Schwarte-Waldhoff at the IMBL, Medical Clinic, Ruhr-University, Bochum, we established cell model systems for the detailed analysis of the DPC4 pathway in pancreatic and colon cancer cells. Supported by German Cancer Foundation (Deutsche Krebshilfe) and the Federal Ministry of Education and Research (BMBF) we have applied functional genomics (SAGE, Micro-Arrays) and proteomics (2D-PAGE, MALDI MS; Prof. H. E. Meyer; MPC) technologies to identify new DPC4 target genes. Some of identifed target genes have already been functionally charcterized others are both at the IMBL and MGO labs in ongoing projects under investigation.