RUB » Bio » BPf » AG Photobiotechnologie » Projects


I. Biochemical and biophysical characterization and de novo design of [FeFe]-hydrogenases and their interaction-partners

  • Structure-function relationsphips in [FeFe]-hydrogenases: Characterization of amino acids involved in proton and electron transfer of H-Cluster coordination as well as characterization of residues essential for the interaction with redox partners
  • Design of O2-tolerant [FeFe]-hydrogenases by site directed mutagenesis and by directed evolution combined with high throughput screening
  • Biochemical and biophysical characterization of hydrogenases, their maturation factors and other O2 sensitive proteins
  • Design and optimization of semi-artificial H2-producing systems
  • Diversity and phylogeny of green algal [FeFe]-hydrogenases

II. Genetic and physiological principles of the anaerobic metabolism of Chlamydomonas reinhardtii

  • Systemic analysis of the anaerobic adaption of Chlamydomonas reinhardtii by generating genome-wide transcript profiles by RNA-sequencing
  • O2-dependent signaling in C. reinhardtii - Identification of O2-sensors, signaling pathways and transcription factors of the hypoxic response
  • Redox and energy homeostasis in Chlamydomonas - characterization of metabolic networks under anaerobic conditions
  • Surviving without oxygen - adaption of O2-dependent biosynthetic pathways in hypoxic Chlamydomonas cultures

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  • Prof. Dr. T. Happe
  • AG Photobiotechnology
    Ruhr-University Bochum
    building: ND room: 2/169
    Universitätsstrasse 150
    44801 Bochum
  • phone: +49 234-32 27026
    fax: +49 234-32 14322
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