Marlena Rohm

MSc. Biochemistry

Marlena Rohm


My research project focuses on the rare neuromuscular disease Morbus Pompe. Using diffusion tensor imaging we could already show a diffusion restriction in leg muscles of LOPD patients prior to fatty degeneration. In order to gain a better understanding of the course of the disease, a long-term study with patients will be conducted. In parallel, a mouse model will be established to gain further insight into the course and potential rescue.


• quantitative imaging methods
• Diffusion Tensor Imaging (DTI - muscles)
• programming in Java and Python
• DNA/RNA: PCR, qPCR, Sequencing, Cloning
• Protein: Laser Microdissection, Western Blot
• Cell culture: Transfection, primary cell culture


since 08/2020
PhD student
Project Group Quantitative Imaging in rare neuro muscular diseases
Prof. Dr. Matthias Vorgerd and Dr. Lara Schlaffke, UK Bergmannsheil

IT Consultant, Java Development
Placement at Allianz Deutschland in Munich
FDM Group GmbH Frankfurt

Research Assistant
Investigating the relevance of NDRG2 for the pathogenesis of the wobbler mouse, a mouse model for ALS
Cytology, Prof. Dr. Carsten Theiss, Ruhr University Bochum

M.Sc. Biochemistry at Ruhr-University Bochum
„Differential expression of the protein NDRG2 as well as the neurodegeneration-associated microRNAs miR-125b-5p and miR-375-3p in the spinal cord of the wobbler mouse“
Cytology, Prof. Dr. Carsten Theiss, Ruhr University Bochum

Student Assistant
Project Group Quantitative Imaging in rare neuro muscular diseases
Dr. Lara Schlaffke, UK Bergmannsheil

Measuring the lifespan of Drosophila melanogaster under various conditions and retinal sectioning
Dr. Manolis Fanto, King’s College London, United Kingdom

B.Sc. Bioanalytic at Hochschule Coburg for applied sciences
„ Mechanism of RNA-toxicity in spinocerebellar ataxia type 12 and type 3” “
“Non-coding RNAs and translational regulation in polyglutamine diseases” – Dr. Sybille Krauss, German Centre for Neurodegenerative Diseases, Bonn,

Intern (practical semester)
Establish PCR and sequencing reaction for qualitative and quantitative analysis of the gene OPA1
“Mitochondrial diseases Laboratory” – Dr. Sandra Jackson, University Hospital Dresden


Rohm M, Markmann M, Forsting J, Rehmann R, Froeling M, Schlaffke L. 3D Automated Segmentation of Lower Leg Muscles Using Machine Learning on a Heterogeneous Dataset. Diagnostics (Basel). 2021 Sep 23;11(10):1747. doi: 10.3390/diagnostics11101747. PMID: 34679445; PMCID: PMC8534967.

Forsting J, Rohm M, Froeling M, Güttsches AK, Vorgerd M, Schlaffke L, Rehmann R. High Inter-Rater Reliability of Manual Segmentation and Volume-Based Tractography in Healthy and Dystrophic Human Calf Muscle. Diagnostics (Basel). 2021 Aug 24;11(9):1521. doi: 10.3390/diagnostics11091521. PMID: 34573863; PMCID: PMC8466691.

Güttsches AK, Rehmann R, Schreiner A, Rohm M, Forsting J, Froeling M, Tegenthoff M, Vorgerd M, Schlaffke L. Quantitative Muscle-MRI Correlates with Histopathology in Skeletal Muscle Biopsies. J Neuromuscul Dis. 2021;8(4):669-678. doi: 10.3233/JND-210641. PMID: 33814461.

Forsting J, Rehmann R, Rohm M, Froeling M, Schlaffke L. Evaluation of interrater reliability of different muscle segmentation techniques in diffusion tensor imaging. NMR Biomed. 2021 Feb;34(2):e4430. doi: 10.1002/nbm.4430. Epub 2020 Nov 20. PMID: 33217106.

Rehmann R, Froeling M, Rohm M, Forsting J, Kley RA, Schmidt-Wilcke T, Karabul N, Meyer-Frießem CH, Vollert J, Tegenthoff M, Vorgerd M, Schlaffke L. Diffusion tensor imaging reveals changes in non-fat infiltrated muscles in late onset Pompe disease. Muscle Nerve. 2020 Oct;62(4):541-549. doi: 10.1002/mus.27021. Epub 2020 Jul 31. PMID: 32654203.

Rohm M, May C, Marcus K, Steinbach S, Theis V, Theiss C, Matschke V. The microRNA miR-375-3p and the Tumor Suppressor NDRG2 are Involved in Sporadic Amyotrophic Lateral Sclerosis. Cell Physiol Biochem. 2019;52(6):1412-1426. doi: 10.33594/000000099. PMID: 31075191.

Schlaffke L, Rehmann R, Rohm M, Otto LAM, de Luca A, Burakiewicz J, Baligand C, Monte J, den Harder C, Hooijmans MT, Nederveen A, Schlaeger S, Weidlich D, Karampinos DC, Stouge A, Vaeggemose M, D'Angelo MG, Arrigoni F, Kan HE, Froeling M. Multi-center evaluation of stability and reproducibility of quantitative MRI measures in healthy calf muscles. NMR Biomed. 2019 Sep;32(9):e4119. doi: 10.1002/nbm.4119. Epub 2019 Jul 17. PMID: 31313867.

Rehmann R, Schlaffke L, Froeling M, Kley RA, Kühnle E, De Marées M, Forsting J, Rohm M, Tegenthoff M, Schmidt-Wilcke T, Vorgerd M. Muscle diffusion tensor imaging in glycogen storage disease V (McArdle disease). Eur Radiol. 2019 Jun;29(6):3224-3232. doi: 10.1007/s00330-018-5885-1. Epub 2018 Dec 17. PMID: 30560358.