Equipment

Microdispenser

Microdispenser

For small-scale micropattern production, piezo microdispensers are indispensable tools for immobilising various molecules (functionalised alkylthiols, proteins, nucleic acids) onto different substrates (glass, gold) in complex patterns. Basically, the spotting solution is filled into a chamber that has a micrometric orifice (nozzle) and a flexible wall. The outer part of the flexible wall is intimately attached to a small bloc of piezoelectric ceramic of which trigging and position are controlled by a specific software. In this way, a defined number of tiny droplets (100 –700 pL) of the spotting solution emitted through a micrometric nozzle are directed towards the predefined regions of the target surface. Not only different geometries but concentration gradients can be easily prepared with this set-up. Spot diameter can range from 50 to 200 µm depending on the microdispenser head, solution viscosity and wetability of the substrate.