Microbial Biochemistry

Publications

2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2004 2003

2020

• Comparison of Proteomic Responses as Global Approach to Antibiotic Mechanism of Action Elucidation
  Senges C H R, Stepanek J J, Wenzel M, Raatschen N, Ay Ü, Märtens Y, Prochnow P, Vázquez Hernández M, Yayci A, Schubert B, Janzing N B M, Warmuth H L, Kozik M, Bongard J, Alumasa J N, Albada B, Penkova M, Lukežič T, Sorto N A, Lorenz N, Miller R G, Zhu B, Benda M, Stülke J, Schäkermann S, Leichert L I, Scheinpflug K, Brötz-Oesterhelt H, Hertweck C, Shaw J T, Petković H, Brunel J M, Keiler K C, Metzler-Nolte N, Bandow J E
  Antimicrob Agents Chemother 65(1): e01373-20
• Redox regulation in host-pathogen interactions: thiol switches and beyond
  Varatnitskaya M, Degrossoli A, Leichert L. I
  Biol Chem online ahead of print 2020 Oct 16
• Regulation of titin-based cardiac stiffness by unfolded domain oxidation (UnDOx)
  Loescher C M, Breitkreuz M, Li Y, Nickel A, Unger A, Dietl A, Schmidt A, Mohamed B A, Kötter S, Schmitt J P, Krüger M, Krüger M, Toischer K, Maack C, Leichert L I, Hamdani N, Linke W A
  Proc Natl Acad Sci U S A. 117(39): 24545-24556.
• The effects of neutrophil-generated hypochlorous acid and other hypohalous acids on host and pathogens
  Ulfig A and Leichert L I
  Cell Mol Life Sci. online ahead of print
• Activation leads to a significant shift in the intracellular redox homeostasis of neutrophil-like cells
  Xie K, Varatnitskaya M, Maghnouj A, Bader V, Winklhofer K F, Hahn S, Leichert L I
  Redox Biol. 28: 101344
• Allicin, a natural antimicrobial defence substance from garlic, inhibits DNA gyrase activity in bacteria
  Reiter J, Hübbers A M, Albrecht F, Leichert L I, Slusarenko A J
  Int J Med Microbiol. 310(1): 151359

2019

• Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote
  Reuter W H, Masuch T, Ke N, Lenon M, Radzinski M, Loi V, Ren G, Riggs P, Antelmann H, Reichmann D, Leichert L I, Berkmen M
  Redox Biol. 26: 101280
• N-chlorination mediates protective and immunomodulatory effects of oxidized human plasma proteins
  Ulfig A, Schulz A V, Müller A, Lupilov N, Leichert L I
  Elife 8: e47395
• The molecular chaperone Hsp33 is activated by atmospheric-pressure plasma protecting proteins from aggregation
  Krewing M, Stepanek JJ, Cremers C, Lackmann JW, Schubert B, Müller A, Awakowicz P, Leichert LIO, Jakob U, Bandow JE.
  J R Soc Interface Epub 16(155): 20180966
• Quantifying changes in the bacterial thiol redox proteome during host-pathogen interaction
  Xie K, Bunse C, Marcus K, Leichert LI.
  Redox Biol. 21: 101087

2018

• Corrigendum: Characterization of ML-005, a Novel Metaproteomics-Derived Esterase
  Sukul P, Lupilov N, Leichert LI.
  Front Microbiol. 9: 2716
• Characterization of ML-005, a Novel Metaproteomics-Derived Esterase
  Sukul P, Lupilov N, Leichert LI.
  Front Microbiol. 22;9: 1925
• Loss of a conserved salt bridge in bacterial glycosyl hydrolase BgIM-G1 improves substrate binding in temperate environments
  Mhaindarkar D, Gasper R, Lupilov N, Hofmann E, Leichert LI
  Commun Biol. 1: 171
• Antimicrobial properties of ternary eutectic aluminum alloys
  Hahn C, Hans M, Hein C, Dennstedt A, Mücklich F, Rettberg P, Hellweg CE,Leichert LI, Rensing C, Moeller R.
  Biometals. 31(5): 759-770
• The mitochondrial oxidoreductase CHCHD4 is present in a semi-oxidized state in vivo.
  Erdogan AJ, Ali M, Habich M, Salscheider SL, Schu L, Petrungaro C, Thomas LW, Ashcroft M, Leichert LI, Roma LP, Riemer J.
  Redox Biol. 17: 200-206.
• Neutrophil-generated HOCl leads to non-specific thiol oxidation in phagocytized bacteria.
  Degrossoli A, Müller A, Xie K, Schneider JF, Bader V, Winklhofer KF, Meyer AJ, Leichert LI.
  Elife 7. pii: e32288.

2017

• Oxidant sensor in the cGMP-binding pocket of PKGIα regulates nitroxyl-mediated kinase activity.
  Donzelli S, Goetz M, Schmidt K, Wolters M, Stathopoulou K, Diering S, Prysyazhna O, Polat V, Scotcher J, Dees C, Subramanian H, Butt E, Kamynina A, Schobesberger S, King SB, Nikolaev VO, de Wit C, Leichert LI, Feil R, Eaton P, Cuello F
  Sci Rep. 7(1): 9938.
• In silico approach to designing rational metagenomic libraries for functional studies.
  Kusnezowa A, Leichert LI.
  BMC Bioinformatics. 18(1): 267.
• A highly conserved redox-active Mx(2)CWx(6)R motif regulates Zap70 stability and activity.
  Thurm C, Poltorak MP, Reimer E, Brinkmann MM, Leichert L, Schraven B, Simeoni L.
  Oncotarget. 8(19): 30805-30816.
• Fluorescence spectroscopy of roGFP2-based redox probes responding to various physiologically relevant oxidant species in vitro.
  Müller A, Schneider JF, Degrossoli A, Lupilova N, Dick TP, Leichert LI.
  Data Brief. 11: 617-627.
• Simple discovery of bacterial biocatalysts from environmental samples through functional metaproteomics.
  Sukul P, Schäkermann S, Bandow JE, Kusnezowa A, Nowrousian M, Leichert LI.
  Microbiome. 5(1): 28.
• Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species.
  Müller A, Schneider JF, Degrossoli A, Lupilova N, Dick TP, Leichert LI.
  Free Radic Biol Med. 106: 329-338.
• Proteome analysis reveals differential expression of proteins involved in triacylglycerol accumulation by Rhodococcus jostii RHA1 after addition of methyl viologen.
  Dávila Costa JS, Silva RA, Leichert LI, Alvarez HM.
  Microbiology. 163(3): 343-354.

2016

• Defects in Mitochondrial Iron-Sulfur Cluster Assembly Induce Cysteine S-Polythiolation on Iron-Sulfur Apoproteins.
  Christ S, Leichert LI, Willms A, Lill R, Mühlenhoff U.
  Antioxid Redox Signal. 25(1): 28-40.
• Sterilization of beehive material with a double inductively coupled low pressure plasma
  Priehn M, Denis B, Aumeier, P, Kirchner WH, Kirchner WH, Awakowicz P, Leichert LI.
  JOURNAL OF PHYSICS D-APPLIED PHYSICS 49(37) DOI: 10.1088/0022-3727/49/37/374002
• Allicin Induces Thiol Stress in Bacteria through S-allylmercapto Modification of Protein Cysteines.
  Müller A, Eller J, Albrecht F, Prochnow P, Kuhlmann K, Bandow JE, Slusarenko AJ, Leichert LI.
  J Biol Chem. Epub ahead of print

2015

• A combined bioinformatics and functional metagenomics approach to discovering lipolytic biocatalysts.
  Masuch T, Kusnezowa A, Nilewski S, Bautista JT, Kourist R, Leichert LI.
  Front Microbiol. 6 1110
• A dielectric barrier discharge terminally inactivates RNase A by oxidizing sulfur-containing amino acids and breaking structural disulfide bonds.
  Lackmann JW, Baldus S, Steinborn E, Edengeiser E, Kogelheide F, Langklotz F, Schneider S, Leichert LIO, Benedikt J, Awakowicz P, Bandow JE.
  Journal of Physics D: Applied Physics 48 494003
• Does the Transcription Factor NemR Use a Regulatory Sulfenamide Bond to Sense Bleach?
  Gray MJ, Li Y, Leichert LI, Xu Z, Jakob U.
  Antioxid Redox Signal. Epub ahead of print Jun 22
• Label-free and redox proteomic analyses of the triacylglycerol-accumulating Rhodococcus jostii RHA1.
  Dávila Costa JS, Herrero OM, Alvarez HM, Leichert LI.
  Microbiology. 161(Pt 3): 593-610.
• Incidence and physiological relevance of protein thiol switches.
  Leichert LI, Dick TP.
  Biol Chem. 396(5): 389-99.

2014

• Activation of RidA chaperone function by N-chlorination.
  Müller A, Langklotz S, Lupilova N, Kuhlmann K, Bandow JE, Leichert LI.
  Nat Commun. 5 5804
• About the dangers, costs and benefits of living an aerobic lifestyle.
  Knoefler D, Leichert LI, Thamsen M, Cremers CM, Reichmann D, Gray MJ, Wholey WY, Jakob U.
  Biochem Soc Trans. 42(4) 917-21
• Extracting iron and manganese from bacteria with ionophores - a mechanism against competitors characterized by increased potency in environments low in micronutrients.
  Raatschen N1, Wenzel M, Leichert LI, Düchting P, Krämer U, Bandow JE.
  Proteomics. 13(8) 1358-70
• Thiol-based redox processes.
  Leichert LI.
  Biochim Biophys Acta. 14 1570-9639

2013

• Redox proteomics uncovers peroxynitrite-sensitive proteins that help Escherichia coli to overcome nitrosative stress.
  Lindemann C1, Lupilova N, Müller A, Warscheid B, Meyer HE, Kuhlmann K, Eisenacher M, Leichert LI.
  J Biol Chem. 288(27) 19698-714
• Nonnative disulfide bond formation activates the σ32-dependent heat shock response in Escherichia coli.
  Müller A1, Hoffmann JH, Meyer HE, Narberhaus F, Jakob U, Leichert LI.
  J Bacteriol. 195(12) 2807-16
• The sulfur carrier protein TusA has a pleiotropic role in Escherichia coli that also affects molybdenum cofactor biosynthesis.
  Dahl JU1, Radon C, Bühning M, Nimtz M, Leichert LI, Denis Y, Jourlin-Castelli C, Iobbi-Nivol C, Méjean V, Leimkühler S.
  J Biol Chem. 288(8) 5426-42

2012

• Quantitative redox proteomics: the NOxICAT method.
  Lindemann C, Leichert LI.
  Methods Mol Biol. 893 387-403
• Redox, haem and CO in enzymatic catalysis and regulation.
  Ragsdale SW1, Yi L, Bender G, Gupta N, Kung Y, Yan L, Stich TA, Doukov T, Leichert LI, Jenkins PM, Bianchetti CM, George SJ, Cramer SP, Britt RD, Jakob U, Martens JR, Phillips GN Jr, Drennan CL.
  Biochem Soc Trans. 40(3) 501-7

2011

• Proteomic methods unravel the protein quality control in Escherichia coli.
  Leichert LI.
  Proteomics. 11(15) 3023-35
• Small RNA-mediated control of the Agrobacterium tumefaciens GABA binding protein.
  Wilms I1, Voss B, Hess WR, Leichert LI, Narberhaus F.
  Mol Microbiol. 80(2) 492-506

2010

• CpeS is a lyase specific for attachment of 3Z-PEB to Cys82 of {beta}-phycoerythrin from Prochlorococcus marinus MED4.
  Wiethaus J1, Busch AW, Kock K, Leichert LI, Herrmann C, Frankenberg-Dinkel N.
  J Biol Chem. 285(48) 37561-9

2009

• Heme regulatory motifs in heme oxygenase-2 form a thiol/disulfide redox switch that responds to the cellular redox state.
  Yi L, Jenkins PM, Leichert LI, Jakob U, Martens JR, Ragsdale SW.
  J Biol Chem. 284(31) 20556-61

2008

• Quantifying changes in the thiol redox proteome upon oxidative stress in vivo.
  Leichert LI, Gehrke F, Gudiseva HV, Blackwell T, Ilbert M, Walker AK, Strahler JR, Andrews PC, Jakob U.
  Proc Natl Acad Sci U S A. 105(24) 8197-202

2007

• Nitrosative stress treatment of E. coli targets distinct set of thiol-containing proteins.
  Brandes N1, Rinck A, Leichert LI, Jakob U.
  Mol Microbiol. 66(4) 901-14

2006

• Global methods to monitor the thiol-disulfide state of proteins in vivo.
  Leichert LI, Jakob U.
  Antioxid Redox Signal. 8(5-6) 763-72
• CoSMoS: Conserved Sequence Motif Search in the proteome.
  Liu XI1, Korde N, Jakob U, Leichert LI.
  BMC Bioinformatics. 7 37

2004

• Protein thiol modifications visualized in vivo.
  Leichert LI, Jakob U.
  PLoS Biol. 2(11) e333. Epub

2003

• Global characterization of disulfide stress in Bacillus subtilis.
  Leichert LI, Scharf C, Hecker M.
  J Bacteriol. 185(6) 1967-75
• Proteomic approach to understanding antibiotic action.
  Bandow JE1, Brötz H, Leichert LI, Labischinski H, Hecker M.
  Antimicrob Agents Chemother. 47(3) 948-55