M. Marek and T. Günther Pomorski (2016).
Assay of flippase activity in proteoliposomes using fluorescent lipid derivatives.
Methods in Molecular Biology 1377: 181–191.
Specific membrane proteins, termed lipid flippases, play a central role in facilitating the movement of lipids across cellular membranes. In this protocol, we describe the reconstitution of ATP-driven lipid flippases in liposomes and the analysis of their in vitro flippase activity based on the use of fluorescent lipid derivatives. Working with purified and reconstituted systems provides a well-defined experimental setup and allows to directly characterize these membrane proteins at the molecular level.