RUB »Analytische Chemie » Dr. Adrian Ruffa



In Press

53. Sokol, K.; Robinson, W.; Oliveira, A.; Warnan, J.; Nowaczyk, M. M.; Ruff, A.; Pereira, I. C. A.; Reisner, E.* Photoreduction of CO2 with a formate dehydrogenase driven by photosystem II using a semi-artificial Z-scheme architecture. J. Am. Chem. Soc. accepted for publication, DOI: 10.1021/jacs.8b10247.

52. Riedel, M.; Wersig, J.; Ruff, A.; Schuhmann, W.; Zouni, A.; Lisdat, F.* A Z-scheme inspired photobioelectrochemical H2O/O2 cell with 1 V open-circuit voltage combining photosystem II and PbS quantum dots. Angew. Chem. Int. Ed., accepted for publication, DOI: 10.1002/anie.201811172.

51. Marković, N.; Conzuelo, F.; Szczesny, J.; García, M. B. G.; Santos, D. H.; Ruff, A.; Schuhmann, W.* An air-breathing carbon cloth-based screen-printed electrode for applications in enzymatic biofuel cells. Electroanalysis, in print, DOI: 10.1002/elan.201800462.

50. Conzuelo, F.*; Ruff, A.*; Schuhmann, W.* Self-powered bioelectrochemical devices. Curr. Opin. Electrochem., in press, DOI: 10.1016/j.coelec.2018.05.010.


49. Szczesny, J.; Markovic, N.; Conzueleo, F.; Zacarias, S.; Pereira, I. A. C.; Lubitz, W.; Plumeré, N.; Schuhmann, W.*; Ruff, A.* A gas breathing hydrogen/air biofuel cell comprising a redox polymer/hydrogenase-based bioanode. Nat. Commun. 2018, 9, 4715.

48. Sokol, K. P.; Robinson, W. E.; Warnan, J.; Kornienko, N.; Nowaczyk, M. M.; Ruff, A.; Zhang, J. Z.; Reisner, E.* Bias-free photoelectrochemical water splitting with photosystem II on a dye-sensitised photoanode wired to hydrogenase, Nat. Energy 2018, 3, 944–951.

47. Jaikaew, W.; Ruff, A.; Khunkaewla, P.; Erichsen, T.; Schuhmann, W.*; Schulte, A.* Robotic microplate voltammetry for real-time hydrogel drug release testing. Anal. Chim. Acta. 2018, 1041, 33–39.

46. Conzuelo, F.*; Markovic, N.; Ruff, A.; Schuhmann, W.* The Open Circuit Voltage in Biofuel Cells: Nernstian Shift in Pseudocapacitive Electrodes. Angew. Chem. Int. Ed. 2018, 57, 13681 –13685. Über die Leerlaufspannung von Biobrennstoffzellen: Nernstverschiebung bei pseudokapazitiven Elektroden. Angew. Chem. 2018, 130, 13870 –13875.

45. Zhao, F.; Hartmann, V.; Ruff, A.; Nowaczyk,M. M.;  Rögner, M.; Schuhmann, W.*; Conzuelo, F.* Unravelling electron transfer processes at photosystem 2 embedded in an Os-complex modified redox polymer. Electrochim. Acta 2018, 290, 451−456.

44. Ruff, A.*; Szczesny, J. Markovic, N.; Conzuelo, F.; Zacarias, S.; Pereira, I. A. C.; Lubitz, W.; Schuhmann, W.* A fully protected hydrogenase/polymer-based bioanode for high-performance hydrogen/glucose biofuel cells. Nat. Commun. 2018, 9, 3675.

43. Lopez, F.; Zerria, S.; Ruff, A.*; Schuhmann, W.* An O2 tolerant polymer/glucose oxidase based bioanode as basis for a self-powered glucose sensor. Electroanalysis (Special Issue, 70th Birthday of Joseph Wang), 2018, 30, 1311−1318.

42. Kopiec, G.; Starzec, K.; Kochana, J.; Campbell, W. H.; Schuhmann, W.; Ruff, A.*; Plumeré, N.* Bioelectrocatalytic and electrochemical cascade for phosphate sensing with up to 6 electrons per analyte molecule. Biosens. Bioelectron., 2018, 117, 501−507.

41. Ruff, A.*§; Qian, X.§; Porfyrakis, K.*; Ludwigs, S. Effect of the type and number of organic addends on fullerene acceptors for n-type electronic devices: redox properties and energy levels. ChemistrySelect, 2018, 3, 5778−5785.

40. Marc Riedel, M.; Parak, W. J.; Ruff, A.; Schuhmann, W.; Lisdat, F.* Light as trigger for biocatalysis: Light as trigger for biocatalysis: Photonic wiring of FAD-dependent glucose dehydrogenase to quantum dot-sensitized inverse opal TiO2 architectures via redox polymers. ACS Catal., 2018, 8, 5212–5220.

39. Marquitan, M.; Bobroswki, T.; Ernst, A.; Wilde, P. Clausmeyer, J.; Ruff, A., Schuhmann, W.*Amperometric Miniaturized Amperometric Glucose Sensors Based on Polymer/ Enzyme Modified Carbon Electrodes in the Sub-Micrometer Scale J. Electrochem. Soc., 2018, 165, G3008−G3014.

38. Teanphonkrang, S.; Janke, S.; Chaiyen, P.; Sucharitakul, J.; Suginta, W.; Khunkaewla, P.; Schuhmann, W.; Ruff, A.*; Schulte, A.* Tuned Amperometric Detection of Reduced β‑ Nicotinamide Adenine Dinucleotide by Allosteric Modulation of the Reductase Component of the p‑Hydroxyphenylacetate Hydroxylase Immobilized within a Redox Polymer Anal. Chem. 2018, 90, 5703−5711.

37. Hartmann, V.; Ruff, A.; Schuhmann, W.; Rögner, M.; Nowaczyk, M. M.* Analysis of Photosystem II Electron Transfer with Natural PsbA-Variants by redox polymer/protein biophotoelectrochemistry. Photosynthetica 2018, 56, 229–235.

36. Bruchlos, K.; Trefz, D.; Hamidi-Sakr, A.; Brinkmann, M.; Heinze, J.; Ruff, A.; Ludwigs, S.* Poly(3-hexylthiophene) revisited – Influence of film deposition on the electrochemical behaviour and energy levels. Electrochim. Acta. 2018, 269, 299–311.

35. Oughli, A. A.; Ruff, A.; Boralugodage, N. P.; Rodríguez-Maciá, P.; Plumeré, N.; Lubitz, W.; Shaw, W. J.*; Schuhmann, W.*; Rüdiger, O.* Dual properties of a hydrogen oxidation Ni-catalyst entrapped within a polymer promote self-defense against oxygen. Nature Commun. 2018, 9, 864.


34. Ruff, A.* Redox polymers in bioelectrochemistry: Common playgrounds and novel concepts. Curr. Opin. Electrochem. 2017, 5/1, 66–73.

33. Jambrec, D.*; Lammers, K.; Bobrowski, T.; Pöller, S.; Schuhmann, W.*; Ruff, A.* Amperometric Detection of dsDNA Using an Acridine-Orange-Modified Glucose Oxidase. ChemPlusChem 2017, 82, 1311–1314.

32. Zhao, F.; Plumeré, N.; Nowaczyk, M. M.; Ruff, A.; Schuhmann, W.*; Conzuelo, F.* Interrogation of a PS1-Based Photocathode by Means of Scanning Photoelectrochemical Microscopy. Small 2017, 5, 1604093.

31. Ruff, A.*; Szczesny, J.; Zacarias, S.; Pereira, I. A. C.; Plumeré, N.; Schuhmann, W.* Protection and Reactivation of the [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough under Oxidative Conditions. ACS Energy Lett. 2017, 2, 964–968.

30. Alsaoub, S.; Ruff, A.*; Conzuelo, F.; Ventosa, E.; Ludwig, R.; Shleev, S.; Schuhmann, W.* An Intrinsic Self-Charging Biosupercapacitor Comprised of a High-Potential Bioanode and a Low-Potential Biocathode. ChemPlusChem 2017, 82, 576–583.

29. Kirres, J.; Schmitt, K.; Wurzbach, I.; Giesselmann, F.; Ludwigs, S.; Ringenberg, M.; Ruff, A.; Baro, A.; Laschat, S.* Tuning liquid crystalline phase behaviour in columnar crown ethers by sulfur substituents. Org. Chem. Front. 2017, 49, 790 –803.

28. Ruff, A.§; Pinyou, P.§; Nolten, M.; Conzuelo, F.; Schuhmann, W.* A Self-Powered Ethanol Biosensor. ChemElectroChem 2017, 4, 890–897.

27. Lopez, F.; Ma, S.; Ludwig, R.; Schuhmann, W.*; Ruff, A.* A Polymer Multilayer Based Amperometric Biosensor for the Detection of Lactose in the Presence of High Concentrations of Glucose. Electroanalysis 2017, 29, 154–161.


26. Sokol, K. P.; Mersch, D.; Hartmann, V.; Zhang, J. Z.; Nowaczyk, M. M.; Rögner, M.; Ruff, A.; Schuhmann, W.; Plumeré, N.*; Reisner, E.* Rational wiring of photosystem II to hierarchical indium tin oxide electrodes using redox polymers. Energy Environ. Sci. 2016, 9, 3698–3709.

25. Masa, J.*; Barwe, S.; Andronescu, C.; Sinev, I.; Ruff, A.; Jayaramulu, K.; Elumeeva, K.; Konkena, B.; Roldan Cuenya, B.; Schuhmann, W.* Low Overpotential Water Splitting Using Cobalt–Cobalt Phosphide Nanoparticles Supported on Nickel Foam. ACS Energy Lett. 2016, 1, 1192–1198.

24. Pinyou, P.§; Ruff, A.§; Pöller, S.; Ma, S.; Ludwig, R.; Schuhmann, W. Design of an Os Complex-Modified Hydrogel with Optimized Redox Potential for Biosensors and Biofuel Cells. Chem. Eur. J. 2016, 22, 5319–5326.

23. Anger, F.; Breuer, T.; Ruff, A.; Klues, M.; Gerlach, A.; Scholz, R.; Ludwigs, S.; Witte, G.; Schreiber, F.* Enhanced Stability of Rubrene against Oxidation by Partial and Complete Fluorination. J. Phys. Chem. C 2016, 120, 5515–5522.

22. Pinyou, P.; Ruff, A.; Pöller, S.; Alsaoub, S.; Leimkühler, S.; Wollenberger, U.; Schuhmann, W.* Wiring of the aldehyde oxidoreductase PaoABC to electrode surfaces via entrapment in low potential phenothiazine-modified redox polymers. Bioelectrochemistry 2016, 109, 24–30.

21. Pinyou, P.; Ruff, A.; Pöller, S.; Barwe, S.; Nebel, M.; Alburquerque, N. G.; Wischerhoff, E.; Laschewsky, A.; Schmaderer, S.; Szeponik, J.; Plumeré, N.; Schuhmann, W.* Thermoresponsive amperometric glucose biosensor. Biointerphases 2016, 11, 11001.



20. Trefz, D.§; Ruff, A.§; Tkachov, R.; Wieland, M.; Goll, M.; Kiriy, A.; Ludwigs, S.* Electrochemical Investigations of the N-Type Semiconducting Polymer P(NDI2OD-T2) and Its Monomer: New Insights in the Reduction Behavior. J. Phys. Chem. C 2015, 119, 22760–22771.

19. Alsaoub, S.§; Barwe, S.§; Andronescu, C.; Pöller, S.; Ruff, A.; Schuhmann, W.* Poly(benzoxazine)s Modified with Osmium Complexes as a Class of Redox Polymers for Wiring of Enzymes to Electrode Surfaces. ChemPlusChem 2015, 80, 1178–1185.

18. Rybalchenko, A. V.; Magdesieva, T. V.*; Brotsman, V. A.; Belov, N. M.; Markov, V. Y.; Ioffe,
I. N.; Ruff, A.; Schuler, P.; Speiser, B.; Heinze, J.; Sidorov, L. N.; Goryunkov, A. A.* The first representative of a new family of the bridgehead-modified difluoromethylenated homofullerenes: electrochemical properties and synthetic availability. Electrochim. Acta 2015, 174, 143–154.

17. Ruff, A.; Heyer, E.; Roland, T.; Haacke, S.; Ziessel, R.; Ludwigs, S.* Electrochemical and optical properties of molecular triads based on triphenylamine, diketopyrrolopyrrole and boron-dipyrromethene. Electrochim. Acta 2015, 173, 847–859.

16. Busseron, E.; Cid, J.-J.; Wolf, A.; Du, G.; Moulin, E.; Fuks, G.; Maaloum, M.; Polavarapu, P.; Ruff, A.; Saur, A.-K.; Ludwigs, S.; Giuseppone, N.* Light-Controlled Morphologies of Self-Assembled Triarylamine-Fullerene Conjugates. ACS nano 2015, 2760–2772.

15. Goll, M.; Ruff, A.; Muks, E.; Goerigk, F.; Omiecienski, B.; Ruff, I.; González-Cano, R. C.; Lopez Navarrete, J. T.; Ruiz Delgado, M. C.; Ludwigs, S.* Functionalized branched EDOT-terthiophene copolymer films by electropolymerization and post-polymerization “click”-reactions. Beilstein J. Org. Chem. 2015, 11, 335–347.

14. Passon, M.§; Ruff, A.§; Schuler, P.; Speiser*, B.; Leis, W. Redox behaviour of some asymmetrically substituted viologens and an alkyl bridged bis-viologen in non-aqueous solvents: a voltammetric and spectroscopic investigation. J. Solid State Electrochem. 2015, 19, 85–101.


13. Roland, T.; Heyer, E.; Liu, L.; Ruff, A.; Ludwigs, S.; Ziessel, R.*; Haacke, S.* A Detailed Analysis of Multiple Photoreactions in a Light-Harvesting Molecular Triad with Overlapping Spectra by Ultrafast Spectroscopy. J. Phys. Chem. C 2014, 118, 24290–24301.

12. Scheuble, M.; Richter, T. V.; Goll, M.; Link, S.; López Navarrete, J. T.; Ruff, A.; Ruiz Delgado, M. C.; Ludwigs, S.* Branched polythiophenes by Ni-catalyzed Kumada coupling. Polym. Chem. 2014, 5, 6824–6833.

11. Erdmann, T.; Back, J.; Tkachov, R.; Ruff, A.; Voit, B.; Ludwigs, S.; Kiriy, A.* Dithienosilole-based all-conjugated block copolymers synthesized by a combination of quasi-living Kumada and Negishi catalyst-transfer polycondensations. Polym. Chem. 2014, 5, 5383 –5390.

10. Passon, M.§; Ruff, A.§; Schuler, P.; Speiser, B.*; Dreiling, I. Stepwise Solid-Phase Synthesis and Solid-State Electrochemistry of Redox-Active Viologen Core/Shell-Structured Modified Silica Materials. ChemElectroChem 2014, 1, 263–280.



9. Link, S. M.; Scheuble, M.; Goll, M.; Muks, E.; Ruff, A.; Hoffmann, A.; Richter, T. V.; López Navarrete, J. T.; Ruiz Delgado, M. C.; Ludwigs, S.* Electropolymerized three-dimensional randomly branched EDOT-containing copolymers. Langmuir 2013, 29, 15463–15473.

8. Samoylova, N. A.; Belov, N. M.; Brotsman, V. A.; Ioffe, I. N.; Lukonina, N. S.; Markov, V. Y.; Ruff, A.; Rybalchenko, A. V.; Schuler, P.; Semivrazhskaya, O. O.; Speiser, B.; Troyanov, S. I.; Magdesieva, T. V.*; Goryunkov, A. A.* [6,6]-Open and [6,6]-closed isomers of C70(CF2): synthesis, electrochemical and quantum chemical investigation. Chem. Eur. J. 2013, 19, 17969–17979.

7. Ruff, A.; Speiser, B.*; Dreiling, I. Redox-active silica nanoparticles. Part 7. Redox behavior of core/shell structured viologen modified silica particles immobilized at paraffin impregnated graphite electrodes. J Electroanal. Chem. 2013, 710, 10–16.

6. Bula, R.; Fingerle, M.; Ruff, A.; Speiser, B.; Maichle-Mössmer, C.; Bettinger, H. F.* Anti-[2.2](1,4)pentacenophane: a covalently coupled pentacene dimer. Angew. Chem. Int. Ed. 2013, 52, 11647–11650; Anti-[2.2](1,4)Pentacenophan, ein kovalent verknüpftes Pentacendimer. Angew. Chem. 2013, 125, 11861–11864.

5. Janisch, J.; Klinkhammer, R.; Ruff, A.; Schäfer, J.; Speiser, B.*; Wolff, C. Two-electron-transfer redox systems. Part 8. Proving the electron stoichiometry for the electrochemical two-electron oxidation of N,N′-bis(ferrocenoyl)-1,2-diaminoethane. Electrochim. Acta 2013, 110, 608–618.

4. Ruff, A.; Schuler, P.; Speiser, B.* Redox-active silica nanoparticles. Part 6. Synthesis and spectroscopic and electrochemical characterization of viologen-modified Stöber silica particles with diameters of approximately 125 nm. J. Solid State Electrochem. 2013, 17, 79–97.


3. Biswas, S.; Müller, M.; Tönshoff, C.; Eichele, K.; Maichle-Mössmer, C.; Ruff, A.; Speiser, B.; Bettinger, H. F.* The Overcrowded Borazine Derivative of Hexabenzotriphenylene Obtained through Dehydrohalogenation. Eur. J. Org. Chem. 2012, 2012, 4634–4639.

2. Plumeré, N.; Ruff, A.; Speiser, B*.; Feldmann, V.; Mayer, H. A. Stöber silica particles as basis for redox modifications: particle shape, size, polydispersity, and porosity. J. Colloid Interface Sci. 2012, 368, 208–219.


1. Janisch, J.; Ruff, A.; Speiser, B*.; Wolff, C.; Zigelli, J.; Benthin, S.; Feldmann, V.; Mayer,
H. A. Consistent diffusion coefficients of ferrocene in some non-aqueous solvents: electrochemical simultaneous determination together with electrode sizes and comparison to pulse-gradient spin-echo NMR results. J. Solid State Electrochem. 2011, 15, 2083–2094.


§ equal contribution
* corresponding author