Tools for
                                                            cytochrome P450 research
 
 

Monoclonal Antibodies for Cytochromes P450






For analytical purposes we offer several monoclonal antibodies for cytochromes P450. They were obtained after immunization of mice with isolated liver microsomal P450 enzymes and subsequent cultivation of selected hybridoma cell lines in bioreactors. Antibodies are purified by affinity chromatography on protein G and their purity is checked by SDS-PAGE.

The following antibodies are offered:
 

 P450-specificity
Ig-type
Species
Price / mg
 CYP1A1
IgG1
 rat
EUR 450,-
CYP1A1/1A2
IgG1
rat
EUR 450,-
CYP2E1
IgG1
human, rat
EUR 450,-
CYP2C11
IgG1
rat
EUR 450,-
CYP2B1
-
rat
EUR 450,-

 

Antibodies are supplied as lyophilized powder and are restored in 50% glycerol according to the enclosed instructions.

For immunoblots, 1 mg of antibody is sufficient for about 300 ml primary antibody solution.

Attention: Use only for in vitro-studies.
 

Applications and Informations
 
 
 
 


Inhibition of liver microsomal EROD-activity by the monoclonal CYP1A1-specific antibody.

Sample: Microsomes of rats treated with 3-methylcholanthrene by intraperitoneal injection. 

EROD-activity of the microsomes (not inhibited):
11.5 nMol resorufin/mg protein * min

                 K       PAH    PAH    3MC   3MC

µg           0.5      0.5        0.5      0.01    0.02
                           microsomal protein

 Immunoblot with rat liver microsomes using the monoclonal CYP1A1-specific antibody.

Samples: Microsomes of

K, untreated rats.
PAH, rats after oral uptake of PAH-mixtures.
3MC, rats treated intraperitoneally with 3-methylcholanthrene.
 

µg                     0.5       2.0        5.0 
                         microsomal protein
 
 

µg              2       2      2      2      2      2
                      microsomal protein
Immunoblot with human liver microsomes using the monoclonal CYP2E1-specific antibody (H5D6).
 
 
 
 
 
 

Immunoblot with rat liver microsomes using the monoclonal CYP2C11-specific antibody (H7E4).
Samples: Microsomes from male and female rats.
From left to right: 
untreated male and female,
dexamethasone treated male and female,
phenobarbital treated male and female.
 
 

 

 

On request:

Heterologously expressed CYP3A - enzymes in insect cell microsomes
(Spodoptera frugiperda and Trichoplusia ni)

Several CYP3A - enzymes of  the rat were expressed in two different insect cell lines. They are supplied as P450 - containing microsomes.
 

P450 - enzyme
remark
CYP3A2
with and without reductase*
CYP3A9
with and without reductase
CYP3A18 
in preparation
CYP3A23
with and without reductase

* NADPH - cytochrome P450 - reductase









Orders and product informations:
 
*RUBITEC
Gesellschaft für Innovation und Technologie der Ruhr-Universität mbH
Gebäude FNO 2
D-44780 Bochum

Germany

 (  +49-234-32-11969
FAX +49-32-14627
: Dietmar.Tappe@ruhr-uni-bochum.de
: Peter.H.Roos@ruhr-uni-bochum.de

CYP1A1, CYP1A2, CYP2C11, CYP2E1, CYP3A2, CYP3A9, CYP3A18, CYP3A23, MAb, monoclonal antibody, heterologous expression