Publications
RUB » Junior Research Group Kourist

Selected Publications

A. Gómez-Baraibar, D. Reichert, C. Mügge, S. Seger, H. Gröger and R. Kourist , A sequential one-pot cascade reaction combining an encapsulated decarboxylase with metathesis for the synthesis of bio-based antioxidants (2016) Ang. Chem., Int. Ed.: in press.



K. Köninger, A. Gomez-Baraibar, C. Mügge, C. Paul., F. Hollmann, M. Nowaczyk and R. Kourist, Recombinant cyanobacteria as tools for asymmetric C=C bond reduction fueled by biocatalytic water oxidation (2016) Ang. Chem., Int. Ed.: DOI: 10.1002/anie.201601200/full

http://onlinelibrary.wiley.com/doi/10.1002/anie.201601200/full

F. Busch, N. Hülsemann, J. Enoki, K. Miyamoto, M. Bocola and R. Kourist, Semiempirical QM/MM calculations reveal a step-wise proton transfer and an unusual thiolate pocket in the mechanism of the racemizing mutant G74C of arylmalonate decarboxylase (2016) Catalysis Science & Technology, DOI: 10.1039/C5CY01964H.

P. Schrepfer, A. Büttner, C. Görner, M. Hertel, F. Wallrapp, W. Eisenreich, V. Sieber, R. Kourist, Thomas Brück* Identification of amino acid networks governing catalysis in the closed complex of class I terpene synthases (2015) Proceedings of the National Academy of Sciences of the USA, DOI: doi/10.1073/pnas.1519680113.

S. K. Gaßmeyer, J. Wetzig, C. Mügge, M. Assmann, J. Enoki, L. Hilterhaus, R. Zuhse, K. Miyamoto, A. Liese and R. Kourist, Arylmalonate decarboxylase-catalyzed asymmetric synthesis of both enantiomers of optically pure flurbiprofen (2015) ChemCatChem DOI: 10.1002/cctc.201501205.

Publications in peer-reviewed journals

2016

A. Gómez-Baraibar, D. Reichert, C. Mügge, S. Seger, H. Gröger and R. Kourist , A sequential one-pot cascade reaction combining an encapsulated decarboxylase with metathesis for the synthesis of bio-based antioxidants (2016) Ang. Chem., Int. Ed.: in press.

J. Maimanakos, J. Chow, S. Gaßmeyer, R. Kourist and W. R. Streit, Sequence-based screening for rare enzymes: new insights into the world of AMDases (2016), Front. Microbiol.: doi:10.3389/fmicb.2016.01332.

J. Enoki, J. Meisborn, A. Müller and R. Kourist (2016). "A multi-enzymatic cascade reaction for the stereoselective production of γ-oxyfunctionalyzed amino acids." Front. Microbiol.: doi: 10.3389/fmicb.2016.00425.

K. Köninger, A. Gomez-Baraibar, C. Mügge, C. Paul., F. Hollmann, M. Nowaczyk and R. Kourist, Recombinant cyanobacteria as tools for asymmetric C=C bond reduction fueled by biocatalytic water oxidation (2016) Ang. Chem., Int. Ed.: DOI: 10.1002/anie.201601200/full

Köninger, K., Zachos, I., Hollmann, F., Kourist, R. Light-driven Enzymatic Decarboxylation. J. Vis. Exp., e53439, doi:10.3791/53439 (2016).

F. Busch, N. Hülsemann, J. Enoki, K. Miyamoto, M. Bocola and R. Kourist, Semiempirical QM/MM calculations reveal a step-wise proton transfer and an unusual thiolate pocket in the mechanism of the racemizing mutant G74C of arylmalonate decarboxylase (2016) Catalysis Science & Technology, DOI: 10.1039/C5CY01964H

P. Schrepfer, A. Büttner, C. Görner, M. Hertel, F. Wallrapp, W. Eisenreich, V. Sieber, R. Kourist, Thomas Brück* Identification of amino acid networks governing catalysis in the closed complex of class I terpene synthases (2015) Proceedings of the National Academy of Sciences of the USA, DOI: doi/10.1073/pnas.1519680113

S. K. Gaßmeyer, J. Wetzig, C. Mügge, M. Assmann, J. Enoki, L. Hilterhaus, R. Zuhse, K. Miyamoto, A. Liese and R. Kourist, Arylmalonate decarboxylase-catalyzed asymmetric synthesis of both enantiomers of optically pure flurbiprofen (2015) ChemCatChem DOI: 10.1002/cctc.201501205.

2015

I. Zachos, S.K. Gaßmeyer, D. Bauer, V. Sieber, F. Hollmann, Robert Kourist, Photobiocatalytic decarboxylation for olefin synthesis (2015) Chemical Communications (IF: 6.8), 51, 1918-1921.

S. K. Gaßmeyer, H. Yoshikawa, J. Enoki, N. Hülsemann, R. Stoll, K. Miyamoto and R. Kourist, STD-NMR based protein engineering of the unique arylpropionate-racemase AMDase G74C (2015), ChemBioChem, 16, 1943-1949.

M. Bartsch, S. K. Gaßmeyer, K. Köninger, K. Igarashi, P. Liauw, N. Dyczmons-Nowaczyk, K. Miyamoto, M. M. Nowaczyk, R. Kourist, Photosynthetic Production of Enantioselective Biocatalysts (2015) Microbial Cell Factories (IF 4.2), 14, 53.

R. Kourist, F. Bracharz, J. Lorenzen, O. Kracht, M. Chovatia, C. Daum, S. Deshpande, A. Lipzen, M. Nolan, R. A. Ohm, I. V. Grigoriev, S. Sun, J. Heitman, T. Brück, and M. Nowrousian, Genomics and transcriptomics of the oil-accumulating basidiomycete yeast Trichosporon oleaginosus: insights into substrate utilization and alternative evolutionary trajectories of fungal mating systems (2015) mBio, 6(4):e00918-15.

T. Masuch, A. Kusnezowa, S. Nilewski, J. T. Bautista, R. Kourist and L. I. Leichert (2015). "A combined bioinformatics and functional metagenomics approach to discovering lipolytic biocatalysts." Front. Microbiol. 6, doi: 10.3389/fmicb.2015.01110

S. Yoshida, J. Enoki, R. Kourist, K. Miyamoto, Engineered hydrophobic pocket of (S)-selective arylmalonate decarboxylase variant by simultaneous saturation mutagenesis to improve catalytic performance (2015) Bioscience, Biotechnology and Biochemistry, doi: 10.1080/09168451.2015.1060844.

S. Yoshida, J. Enoki, R. Hemmi, R. Kourist, N. Kawakami, and K. Miyamoto, Draft genome sequence of Bordetella bronchiseptica KU1201, the first isolation source of arylmalonate decarboxylase (2015) Genome Announcements, 3, e00373-15.

2013

R. Wada, T. Kumon, R. Kourist, H, Ohta, D. Uemura, S. Yoshida, K. Miyamoto, Thermally driven asymmetric domino reaction catalyzed by a thermostable esterase and its variants (2013) Tetrahedron Letters, 15, 1921-1923.

2012

HH. Brundiek, A.S. Evitt, R. Kourist, U.T. Bornscheuer, Creation of lipase highly selec¬tive for trans fatty Acids by protein engineering (2012) Angewandte Chemie International Edition, 51, 412-414.

H. Brundiek, S.K. Padhi, A. Evitt, R. Kourist, U.T. Bornscheuer, Altering the scissile fatty acid binding site of Candida antarctica lipase A by protein engineering for the selective hydrolysis of medium chain fatty acids (2012) European Journal of Lipid Science and Technology, 114, 1148-1153.

H. Brundiek, S. Sass, A.S. Evitt, R. Kourist, U.T. Bornscheuer, The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans fatty acid selectivity (2012) Applied Microbiology and Biotechnology, 94, 141-150.

J. Rehdorf,G.A. Behrens, G.S. Nguyen, R. Kourist, U.T. Bornscheuer, Pseudomonas putida esterase contains a GGG(A)X-motif confering activity for the kinetic resolution of tertiary alcohols (2012) Applied Microbiology and Biotechnology, 1119-26.

2011

R. Kourist, Y. Miyauchi, D. Uemura, K. Miyamoto, Engineering the promiscuous racemase activity of arylmalonate decarboxylase (2011) Chemistry – A European Journal, 17, 557-563.

Y. Miyauchi, R. Kourist, D. Uemura, K. Miyamoto, Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution (2011), Chemical Communications, 47, 7503-7505.

S. Herter, G.S. Nguyen, M.L. Thompson, F. Steffen-Munsberg, F. Schauer, U.T. Bornscheuer, R. Kourist*, Comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries (2011) Applied Microbiology and Biotechnology, 90, 929-939.

G.S. Nguyen, M.L. Thompson, G. Grogan, U.T.Bornscheuer, R. Kourist*, Identification of novel esterases for the synthesis of sterically demanding chiral alcohols by sequence-structure guided genome mining (2011) Journal of Molecular Catalysis B: Enzymatic, 70, 88–94.

K. Engelmark Cassimjee, R. Kourist, G, D. Lindberg, M. Wittrup Larsen, N. H. Thanh, M. Widersten, U. T. Bornscheuer and P. Berglund, One-Step Enzyme Extraction and Immobilisation for Biocatalysis Applications (2011) Biotechnology Journal, 6, 463–469.

2010

R. Kourist, H.Jochens, S. Bartsch, S. K. Padhi, M. Gall, D. Böttcher R. Kuipers, H.-J. Joosten, U. T. Bornscheuer, ABHDB – A molecular class specific information system as a tool for the protein engineering of /-fold hydrolases – principle and applications (2010) ChemBioChem, 11, 1635-1643.


Bassegoda, G.S. Nguyen, M. Schmidt, R. Kourist, P. Diaz, U. T. Bornscheuer, Rational protein Design of Paenibacillus barcinonensis esterase EstA for kinetic resolution of tertiary alcohols (2010) ChemCatChem, 2, 962-967.

G.S. Nguyen, R. Kourist, M. Paravidino, A. Hummel, J. Rehdorf, R. V. A. Orru, U. Hanefeld and U. T. Bornscheuer (2010) An enzymatic toolbox for the kinetic resolution of 2-(pyridinyl)but-3-yn-2-ols and tertiary cyanohydrins (2010) European Journal of Organic Chemistry, 14, 2753-2758.

M. Gall, R. Kourist, M. Schmidt, U. T. Bornscheuer, The role of the GGGX-motif on activity and enantioselectivity of pig liver esterase towards tertiary alcohols (2010) Biocatalysis and Biotransformation, 28, 201-208.

M. Theurer, P. Fischer, A. Baro, G.S. Nguyen, R. Kourist, U.T. Bornscheuer, S. Laschat, Formation of chiral tertiary homoallylic alcohols via Evans aldol reaction or enzymatic resolution and their influence on the Sharpless asymmetric dihydroxylation (2010) Tetrahedron, 66, 3814-3823.

2009

E. Fernandez-Alvaro, R. Kourist, J. Winter, D. Böttcher, K. Liebeton, Jürgen Eck, Christian Naumer, Karl-Erich Jäger, Wolfgang Streit, U. T. Bornscheuer, Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases (2009) Microbial Biotechnology (IF 3.0), 3, 59-64.

M. Wiggers, J. Holt, R. Kourist, S. Bartsch, I. Arends, A. J. Minnaard, U. T. Bornscheuer, U. Hanefeld, Probing the enantioselectivity of Bacillus subtilis esterase BS2 for tert. alcohols (2009) Journal of Molecular Catalysis B: Enzymatic (IF 2.8), 60, 82-86.

R. Kourist, H. Brundiek, U. T. Bornscheuer, Protein engineering and discovery of lipases (2009) European Journal of Lipid Science and Technology, 112, 64-74.

2008

S. Bartsch, R. Kourist, and U. T. Bornscheuer, Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esterase (2008) Angewandte Chemie International Edition, 47, 8, 1508-1511.

R. Kourist, G.S. Nguyen, D. Strübing, D. Böttcher, K. Liebeton, C. Naumer, J. Eck, U. T. Bornscheuer, Hydrolase-catalysed stereoselective preparation of protected α,α-dialkyl-α-hydroxycarboxylic acids (2008) Tetrahedron: Asymmetry, 19, 5, 1839-1843.

2007

R. Kourist, S. Bartsch, L. Fransson, K. Hult, and U. T. Bornscheuer, Understanding promiscuous amidase activity of an esterase from Bacillus subtilis (2007) ChemBioChem, 9, 1, 67-69.

R. Kourist, S.H. Krishna, J. S. Patel, F. Bartnek, T. S. Hitchman, D. P. Weiner and U. T. Bornscheuer, Identification of a metagenome-derived esterase with high enantioselectivity in the kinetic resolution of arylaliphatic tertiary alcohols (2007) Organic and Biomolecular Chemistry, 5, 20, 3310-3313.

R. Kourist, S. Bartsch, and U. T. Bornscheuer, Highly enantioselective synthesis of arylaliphatic tertiary alcohols using mutants of an esterase from Bacillus subtilis (2007) Advanced Synthesis and Catalysis, 349, 8-9, 1393-1398.

B. Heinze, R. Kourist, L. Fransson, K. Hult and U. T. Bornscheuer, Highly enantioselective kinetic resolution of two tertiary alcohols using mutants of an esterase from Bacillus subtilis (2007) Protein Engineering Design & Selection, 20, 125-131.

M. Schmidt, E. Henke, B. Heinze, R. Kourist, A. Hidalgo, Uwe T. Bornscheuer, A versatile esterase from Bacillus subtilis: cloning, expression, characterization, and its application in biocatalysis (2007) Biotechnology Journal, 2, 2, 249-253.

2005

R. Kourist, J. Gonzales-Sabín, R. Liz Guiral, F. Rebolledo, Kinetic resolution of 1-biaryl- and 1-(pyridylphenyl)alkan-1-ols catalysed by the lipase B from Candida antarctica, (2005) Advanced Synthesis and Catalysis, 347, 5, 695-702.

Reviews

R. Kourist, A New Class of Enzymes Discovered: Non-heme Oxidase Produces Medium-chain 1-Alkenes (2015) Angew. Chem., Int. Ed., (IF: 11.3), 54, 4156-4158.

R. Kourist, J.-K. Guterl, K. Miyamoto, V. Sieber, Enzymatic decarboxylation—an emerging reaction for chemicals production from renewable resources (2014) ChemCatChem (IF: 5.0), 6, 689-701.

T. Brück, R. Kourist, B. Loll, Production of Macrocyclic Sesqui‐and Diterpenes in Heterologous Microbial Hosts: A Systems Approach to Harness Nature’s Molecular Diversity (2014) ChemCatChem (IF: 5.0), 6, 1142-1165.

R. Kourist*, Pablo Domínguez de María, Kenji Miyamoto, Biocatalytic approaches for a sustainable synthesis of profens – recent trends and developments (2011), Green Chemistry (IF 6.8), 2607-2618.

R. Kourist, U.T. Bornscheuer, Biocatalytic synthesis of optically active tertiary alcohols (2011) Applied Microbiology and Biotechnology (IF 3.8), 91, 505-517.

R. Kourist, P. Domínguez de María and U. T. Bornscheuer, Enzymatic synthesis of optically active tertiary alcohols: expanding the biocatalysis toolbox (2008) ChemBioChem, 9, 4, 491-498.

R. Kourist, M. Höhne, U. T. Bornscheuer, Protein Design: Im Spannungsfeld zwischen gerichteter Evolu¬tion und rationalen Design (2009) Chemie in unserer Zeit, 43, 132-14.

Bookchapters

R. Kourist, S. Gassmeyer, (2015) Biocatalysis and Protein Engineering, in: Biotechnology. Editors: U. Kück, Nicole Frankenberg-Dinkel.

R. Kourist, U.T. Bornscheuer, Biocatalysis (2014), in: Applied Homogenous Catalysis with Organometallic Compounds: A Comprehensive Handbook in Three Volumes. Editors: B. Cornils, W.A. Hermann, M. Beller, A. Renken, R. Paciello.

K. Miyamoto, S. Yoshida, R. Kourist and H. Ohta, Asymmetric Decarboxylation of Arylmalonates and Racemization of Profens by Arylmalonate Decarboxylase and its Variants (2012) in: C–C Bond Formation and Decarboxylation, Practical Methods in Biocatalysis. John Whittall, Peter Sutton (Editors), Wiley VCH

R. Kourist*, S. Herter, U.T. Bornscheuer (2015) Identification of new biocatalysts for the enantioselective conversion of tertiary alcohols in "Practical Methods in Biocatalysis and Biotransformations" Editor: Wolfgang Kroutil. in press

R. Kourist, S. Gassmeyer, (2015) Biocatalysis and Protein Engineering, in: Biotechnology. Editors: U. Kück, Nicole Frankenberg-Dinkel.

Patents

Verfahren zur Herstellung von 2-Aryl-2-Allyl-malonsäuren, Patent application Nr. P04224, April 2014.

Bornscheuer, U., Brundiek, H., Evitt, A., Sass, S., Boenisch, F., Kourist, R. (2013), Polynucleotides, vectors, and host cells expressing improved lipase variants, US Patent Application, US 20130196411.

Bornscheuer, U., Brundiek, H., Evitt, A., Sass, S., Boenisch, F., Kourist, R. (2013), Reduced-fat foodstuffs and cooking oils, and methods for making the same, US Patent Application, US 20130171321.

Bornscheuer, U., Brundiek, H., Evitt, A., Sass, S., Boenisch, F., Kourist, R. (2012), Lipase variants, US Patent Application, US 20120276247.

Bornscheuer, U., Brundiek, H., Evitt, A., Sass, S., Boenisch, F., Kourist, R. (2012), Lipase variants having increased enzyme specificity or enhanced trans-selectivity, and methods of use, PCT Patent Application, WO 2012146935.

Bornscheuer, U., Brundiek, H., Evitt, A., Sass, S., Boenisch, F., Kourist, R. (2012), Lipase variants of ustilaginaceae, PCT Patent Application, WO 2012146937


Other Publications

Buchbesprechung: Science of Synthesis compendium Biocatalysis for Organic Synthesis edited by Kurt Faber, Wolf-Dieter Fessner and Nicolas J. Turner. Angewandte Chemie International Edition, 2015.

K. Köninger, I. Zachos, M. Grote, F. Hollmann, R. Kourist, Light-catalyzed H2O2 generation is used to drive the decarboxylation of fatty acids (2015) Journal of Visualized Experiments, in press

R. Kourist, F. Hollmann, G.S. Nguyen, Lipases as Sustainable Biocatalysts for the Sustainable Industrial Production of Fine Chemicals and Cosmetics, (2014) JSM Biotechnology and Bioengineering, 2, 1029

Licht veredelt nachwachsende Rohstoffe – Interview mit dem Deutschlandfunk am 16.07.2015. http://www.deutschlandfunk.de/biologische-synthese-maschine-licht-veredelt-nachwachsende.676.de.html?dram%3Aarticle_id=325683

R. Kourist, K. Miyamoto, Protein engineering of Arylmalonate Decarboxylase G74C, an artificial racemase (2011) BioTech International 23, 12573.