Dog gPRA

Diagnostics

Direct gene diagnosis possible for gPRA in Sloughi dogs

We are offering direct gene diagnostics for gPRA, which is transmitted autosomal recessively in Sloughi dogs. Since the mutation is directly demonstrable by PCR-based tests, the diagnostic certainty is virtually 100%. For breeding purposes heterozygous individuals (asymptomatic gene carriers) can be distinguished reliably from normal (homozygous) genotypes and from affected animals (homozygously mutant). For further details on the discovered mutation see Dekomien et al., 2000 in the sientific journal Cytogenetics and cell genetics.

For ordering of the DNA test (€ 56.- per case) see the attached sheet.

Genetic Diagnostics Sloughi

Direct gene diagnosis possible for gPRA in Schapendoes dogs

Genetic linkage analyses had identified a marker linked to the gPRA locus for Schapendoes dogs (Lippmann et al., 2007). One allele of this marker generates information about the gPRA status of the analysed Schapendoes. Meanwhile, we identified the gPRA causative mutation in a candidate gene of the marker linked region and established a direct mutation test since 1st October 2008. This test (€ 66.- per case) analyses the mutation directly in the gene which is homozygous in affected Schapendoes dogs.

Because of the distance between diagnostic marker and the identified mutation of about 1.6 Mb there is a small theoretical chance for false negative, respectively, positive results in fact of recombination events. In the Schapendoes breed we identified recombination events between marker and mutation in ?1% of all analysed dogs whereby only the b allele is concerned. Thus, we recommend still to order the direct mutation test for all those Schapendoes dogs which had been diagnosed at least with one b allele by the indirect marker test.

Therefore, it is sufficient for Schapendoes owners with indirectly tested dogs to send the order form [PDF] for the direct DNA test. The results will be sent back to the denoted address together with an invoice of 30.- €. (The shipping of further blood samples and a copy of the pedigree is not required for Schapendoes dogs which had already been tested by the indirect marker test.)

Genetic Diagnostics Schapendoes

Molecular genetic risk test for gPRA in Irish Glen of Imaal Terrier available!

After 3 years of intense research, we have identified a mutation in the genome of many Glen of Imaal Terriers, that – in homozygous state – is closely associated with gPRA (Kropatsch et al. 2010). The age of gPRA onset in Glens appears quite variable. The price (€ 70) includes DNA preparation, typing and expertise to be delivered to the owner per mail. For ordering of the risk test see the attached sheet.

Genetic Diagnostics Glen of Imaal Terrier