Prof. Dr. Stefan Wiese, Group for Molecular Cell Biology

Stefan Wiese

Research Programme:

A major interest of the Research group for Molecular Cell Biology is the analysis of pathomechanisms leading to motor neuron cell death or axonal degeneration. In the past years the research focus has been on the different signalling cascades that are potentially involved in these mechanisms (Wiese et al., 1999b; Wiese et al., 2001; Holtmann et al., 2005; Schweizer et al., 2002; Götz et al., 2005). During this work, key regulator proteins in the mechanisms of motor neuron survival have been identified and new insights into protein-protein interactions have been described. The group has moved to Bochum recently with great expertise in the culture system of embryonic motoneurons, sensory neurons and neural stem cells from embryonic forebrain. Motoneuron cultures have been performed from different species including chick, rat and mouse. Intensive studies have been performed on the survival and cell death of cultured motor neurons and sensory neurons in chicken and various mouse mutants (Banks et al., 2005; Holtmann et al., 2005; Wiese et al., 2001; Wiese et al., 1999a; Camarero et al., 2006; Bömmel et al., 2002) as well as on the analysis of neural stem cells (Götz et al., 2005; Harder et al., 2004). And finally, in vitro studies have been also followed up by in vivo studies using the facial nerve lesion model in mice.

PHD projects

  • Differentiation of Spinal cord-derived neural stem cells
  • Mechanisms for Neuronal damage in ALS models
  • Involvement of microglia in axon degeneration and cell death of motoneurons
  • Analysis of extra cellular matrix components in astrocyte – motoneuron co-cultures: Role for neuronal damage repair in the spinal cord

Student courses

  • Analysis of nerve cells, Practical course
  • Culture and analysis of embryonic motoneurons