Publications within BIOENERGY


S. Alsaoub, A. Ruff, F. Conzuelo, E. Ventosa, R. Ludwig, S. Shleev, W. Schuhmann. “An Intrinsic Self-Charging Biosupercapacitor Comprised of a High-Potential Bioanode and a Low-Potential Biocathode” ChemPlusChem 82 (2017) 576-583.

P. Bollella, L. Gorton, R. Ludwig, R. Antiochia. “Third Generation Glucose Biosensor based on Cellobiose Dehydrogenase Immobilized on Electrodeposited Gold Nanoparticles Modified Electrode: Characterization and Application in Human Saliva” Sensors 17 (2017) 1912-1925.

P. Bollella, R. Ludwig, L. Gorton. “Cellobiose Dehydrogenase: Insights on the Nanostructuration of Electrodes for Improved Development of Biosensors and Biofuel Cells” Appl. Mater. Today 9 (2017) 319–332.

Bollella, F. Mazzei, G. Favero, G. Fusco, R. Ludwig, L. Gorton, R. Antiochia. "Improved DET communication between cellobiose dehydrogenase and a gold electrode modified with a rigid self-assembled monolayer and green metal nanoparticles: The role of an ordered nanostructuration" Biosens Bioelectron 88 (2017) 196–203.

L. Darus, R. V. Lemos, T. Sadakane, P. Ledezma, S. Tsujimura, I. Osadebe, D. Leech, L. Gorton, S. Freguia. "Redox-Polymers Enable Uninterrupted Day/Night Photo-Driven Electricity Generation in Biophotovoltaic Devices" J Electrochem Soc, 164 (2017) H3037-H3040.

S. Draminska, R. Bilewicz. “Bienzymatic mediatorless sensing of total hydrogen peroxide withcatalase and multi-copper enzyme co-adsorbed at carbonnanotube-modified electrodes” Sensors and Actuators B 248 (2017) 493–499.

F. A. Al-Lolage, M. Meneghello, S. Ma, R. Ludwig, P.N. Bartlett. “A Flexible Method for the Stable, Covalent Immobilization of Enzymes at Electrode Surfaces” ChemElectroChem 4 (2017) 1528–1534.

C. Gonaus, D. Maresch, K. Schropp, P. O’Conghaile, D. Leech, L. Gorton, C. Peterbauer. „Analysis of Agaricus meleagris pyranose dehydrogenase N-glycosylation sites and performance of partially non-glycosylated enzymes” Enzyme Microb. Technol. 99 (2017) 57–66.

E. Gonzalez-Arribas, O. Aleksejeva, T. Bobrowski, M.D. Toscano,L. Gorton, W. Schuhmann, S. Shleev. "Solar biosupercapacitor" Electrochem Comm, 74 (2017) 9-13.

M.J. Gonzalez-Guerrero, F.J. del Campo, J.P. Esquivel, D. Leech, N. Sabate. "Paper-based microfluidic biofuel cell operating under glucose concentrations within physiological range" Biosens Bioelectron 90 (2017) 475-480.

E. Gonzalez-Arribas, T. Broboswky, C. Di Bari, K. Sliozberg. R. Ludwig, M. D. Toscano, A.L. De Lacey, M. Pita, W. Schuhmann, S. Shleev. “Transparent, mediator- and membrane-free enzymatic fuel cell based on nanostructured chemically modified indium tin oxide electrodes” Biosens Bioelectron 97(2017) 46-52.

V. Grippo,] J. Pawłowska, J.F. Biernat, R. Bilewicz. "Synergic Effect of Naphthylated Carbon Nanotubes and Gold Nanoparticles on Catalytic Performance of Hybrid Films Containing Bilirubin Oxidase for the Dioxygen Reduction" Electroanalysis 29 (2017) 103-109.

K. Hasan, V. Grippo, E. Sperling, M. A. Packer, D. Leech, L. Gorton. "Evaluation of Photocurrent Generation from Different Photosynthetic Organisms" ChemElectroChem 4 (2017) 412–417.

D. Jain, A. Karajic, M. Murawska, B. Goudeau, S. Bichon, S. Gounel, N. Mano, A. Kuhn, P. Barthelemy., "Low-Molecular-Weight Hydrogels as New Supramolecular Materials for Bioelectrochemical Interfaces" ACS Appl Mat Interf 9 (2017) 1093-1098.

H. Kanso, M.B. González-García, L- Fernández-Llano, S. Ma, R. Ludwig, P. Fanjul-Bolado, and David Hernández-Santos. “Novel thin layer flow-cell screen-printed graphene electrode for enzymatic sensors” Biosens. Bioelectron. 93 (2017) 298-304

H. Kanso, M.B. Gonzalez Garcia, S. Ma, R. Ludwig, P. Fanjul Bolado, D. Hernandez Santos. "Dual Biosensor for Simultaneous Monitoring of Lactate and Glucose Based on Thin-layer Flow Cell Screen-printed Electrode" Electroanalysis 29 (2017) 87-92.

M. Kizling, M. Dzwonek, B. Olszewski, P. Bącal, Ł. Tymecki, A. Więckowska, K. Stolarczyk, R. Bilewicz. “Reticulated vitreous carbon as a scaffold for enzymatic fuel cell designing” Biosensors and Bioelectronics 95 (2017) 1-7.

F. Lopez, S. Ma, R. Ludwig, W. Schuhmann, A. Ruff, "A Polymer Multilayer Based Amperometric Biosensor for the Detection of Lactose in the Presence of High Concentrations of Glucose" Electroanalysis 29 (2017) 154-161.

S. Ma, M. Preims, F. Piumi, L. Kappel, B. Seiboth, E. Record, D. Kracher, R. Ludwig. „Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems” Microbial Cell Factories 16 (2017) 37.

I. Malytska, C. Mézière, M. Kielar, L. Hirsch, G. Wantz, N. Avarvari, A. Kuhn, L. Bouffier. „Bipolar electrochemistry with organic single crystals for wireless synthesis of metal−organic Janus objects and asymmetric photovoltage generation” J. Phys. Chem. C 121 (2017) 12921−12927.

G. Mark, E. Suraniti, J. Roche, H. Richter, A. Kuhn, N. Mano and P. Fischer. “On-Chip Enzymatic Microbiofuel Cell-Powered Integrated Circuits” Lab on Chip 17 (2017) 1761-1768.

G. Pankratova, K. Hasan, D. Leech, L. Hederstedt, L. Gorton. "Electrochemical wiring of the Gram-positive bacterium Enterococcus faecalis with osmium redox polymer modified electrodes" Electrochem Comm, 75 (2017) 56-59.

G. Pankratova, D. Pankratov, K Hasan, H.-E. Åkerlund, P.-Å. Albertsson, D. Leech, S. Shleev, L. Gorton. “Supercapacitive Photobioanodes and Bio-solar Cells: a Novel Approach for Solar Energy Harnessing” Adv. Enery Mater. 7 (2017) 1602285.

A. Ruff, P. Pinyou, M. Nolten, F. Conzuelo, W. Schuhmann. „A Self-Powered Ethanol Biosensor” ChemElectroChem 4 (2017) 890-897.

A. Ruff, J. Szczesny, S. Zacarias, I.A.C. Pereira, N. Plumere, W. Schuhmann. “Protection and Reactivation of the [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough under Oxidative Conditions” ACS Energy Letters 2 (2017) 964-968.

T. Siepenkoetter, U. Salaj-Kosla, E. Magner. “The Immobilization of Fructose Dehydrogenase on Nanoporous Gold Electrodes for the Detection of Fructose” ChemElectroChem 4 (2017) 905-12.

T. Siepenkoetter, U. Salaj-Kosla, X. Xiao, P. O’Conghaile, M. Pita, R. Ludwig, E. Magner. “Immobilisation of redox enzymes on nanoporous gold electrodes: applications in biofuel cells” ChemPlusChem 82 (2017) 553-560.

C. Tapia, R.D. Milton, G. Pankratova, S.D. Minteer, H.E. Akerlund, D. Leech, A. L. De Lacey, M. Pita, L. Gorton. "Wiring of Photosystem I and Hydrogenase on an Electrode for Photoelectrochemical H2 Production by using Redox Polymers for Relatively Positive Onset Potential" ChemElectroChem 4 (2017) 90-95.

M. Tavahodi, R. Ortiz, C. Schulz, A. Ekhtiari, R. Ludwig, B. Haghighi, L. Gorton. “Direct Electron Transfer of Cellobiose Dehydrogenase on Positively Charged Polyethyleneimine Gold Nanoparticles” ChemPlusChem 82 (2017) 546-552.

XX. Xiao, P. O'Conghaile, D. Leech, R. Ludwig, E. Magner. "A symmetric supercapacitor/biofuel cell hybrid device based on enzyme-modified nanoporous gold: An autonomous pulse generator" Biosens Bioelectron 90 (2017) 96-102.

XX. Xiao, P. O'Conghaile, D. Leech, R. Ludwig, E. Magner. “An oxygen-independent and membrane-less glucose biobattery/supercapacitor hybrid device” Biosens Bioelectron 90 (2017) 421-427.



C. Di Bari, A. Goni-Urtiaga, M. Pita, S. Shleev, M.D. Toscano, R. Sainz, A.L. De Lacey. "Fabrication of high surface area graphene electrodes with high performance towards enzymatic oxygen reduction" Electrochim. Acta 191 (2016) 500-509.

C. Di Bari, S. Shleev, A.L. De Lacey, M. Pita. "Laccase-modified gold nanorods for electrocatalytic reduction of oxygen" Bioelectrochemistry 107 (2016) 30-36.

M. Falk, R. Sultana, M. Swann, A.R. Mount, N. J. Freeman. "Nanoband array electrode as a platform for high sensitivity enzyme-based glucose biosensing” Bioelectrochemistry 112 (2016) 100–105.

E. González-Arribas, D. Pankratov, S. Gounel, N. Mano, Z. Blum, S. Shleev. "Transparent and capacitive bioanode based on specifically engineered glucose oxidase" Electroanalysis 28 SI (2016) 1290-1297.

A.Karajic, S. Reculusa, S. Ravaine, N. Mano, A. Kuhn, "Miniaturized Electrochemical Device from Assembled Cylindrical Macroporous Gold Electrodes" ChemElectroChem 3 (2016) 2031-2035.

M. Kizling, P. Biedul, D. Zabost,K. Stolarczyk, R. Bilewicz. "Application of Hydroxyethyl Methacrylate and Ethylene Glycol Methacrylate Phosphate Copolymer as Hydrogel Electrolyte in Enzymatic Fuel Cell" Electroanalysis 28 (2016) 2444-2451.

M. Kizling, K. Stolarczyk, P. Tammela, Z. Wang, L. Nyholm, J. Golimowski, R. Bilewicz. “Bioelectrodes based on pseudocapacitive cellulose/polypyrrole composite improve performance of biofuel cell” Bioelectrochemistry 111 (2016) 184-190.

D. Majdecka, R. Bilewicz. "Nanostructuring carbon supports for optimal electrode performance in biofuel cells and hybrid fuel cells" J. Solid State Electrochem. 20 (2016) 949-955.

D.M. Mate, D. Gonzalez-Perez, I. Mateljak, P.Gomez de Santos, A.I. Vicente, M. Alcalde. “The pocket manual of directed evolution: Tips and tricks. In: Biotechnology of Microbial Enzymes: Production, Biocatalysis and Industrial Applications”. Brahmachari, Demain and Adrio Eds. Elsevier. (2016) 185-214.

P. Molina-Espeja, J. Viña-Gonzalez, B.J. Gomez, J. Martin-Diaz, E. Garcia-Ruiz, M. Alcalde. ”Beyond the outer limits of nature by directed evolution” Biotechnol Adv 34 (2016) 754-767.  

D. Pankratov, F. Conzuelo, P. Pinyou, S. Alsaoub, W. Schuhmann, S. Shleev. "A Nernstian Biosupercapacitor" Angew. Chem., Int. Ed. 55 (2016) 15434-15438.

P. Pinyou, A. Ruff, S. Pöller, S. Ma, R. Ludwig, W. Schuhmann. "Design of an Os Complex-Modified Hydrogel with Optimized Redox Potential for Biosensors and Biofuel Cells" Chem. Eur. J. 15 (2016) 5319-5326.

C. Schulz, R. Kittl, R. Ludwig, L. Gorton. "Direct Electron Transfer from the FAD Cofactor of Cellobiose Dehydrogenase to Electrodes" ACS Catalysis 6 (2016) 555-563.

T. Siepenkoetter, U. Salaj-Kosla, X. Xiao, S. Belochapkine, E. Magner. "Nanoporous Gold Electrodes with Tuneable Pore Sizes for Bioelectrochemical Applications" Electroanalysis 28 (2016) 2415-2423.

S. Shleev, V. Andoralov, D. Pankratov, M. Falk, O. Aleksejeva, Z. Blum. "Oxygen Electroreduction versus Bioelectroreduction: Direct Electron Transfer Approach" Electroanalysis 28 (2016) 2270-2287.

X. Xiao, P.Si, E. Magner. "An overview of dealloyed nanoporous gold in bioelectrochemistry" Bioelectrochemistry 109 (2016) 117-126.


M. Alcalde. "Engineering the ligninolytic enzyme consortium" Trends in Biotechnology33 (2015) 155-162.

S. Barwe, C. Andronescu, S. Pöller, W.Schuhmann. "Codeposited Poly(benzoxazine) and Os-Complex Modified Polymethacrylate Layers as Immobilization Matrix for Glucose Biosensors" Electroanalysis 27 (2015) 2158-2163.

C. Gutierrez-Sanchez, S. Shleev, A. L. De Lacey, M. Pita. "Third-generation oxygen amperometric biosensor based on Trametes hirsuta laccase covalently bound to graphite electrode" Chemical Papers, 69 (1) (2015) 237–240.

H. Hamidi, K. Hasan, S.C. Emek, Y. Dilgin, H.E. Akerlund, P.A. Albertsson, D. Leech, L. Gorton. "Photocurrent Generation from Thylakoid Membranes on Osmium-Redox-Polymer-Modified Electrodes" ChemSusChem 8 (2015) 990-993.

K. Hasan, E. Çevik, E. Sperling, M.A. Packer, D. Leech, L. Gorton. “Photoelectrochemical Wiring of Paulschulzia pseudovolvox (Algae) to Osmium Polymer Modifi ed Electrodes for Harnessing Solar Energy” Adv Energy Mater (2015) 1501100.

K. Hasan, V.R. Reddy, V. Eßmann, K. Górecki, P. Ó'Conghaile, W. Schuhmann, D. Leech, C. Hägerhäll, Lo Gorton. "Electrochemical communication between electrodes and Rhodobacter capsulatus grown in different metabolic modes" Electroanalysis 27 (2015) 118-127.

A. Karajic, S. Reculusa, M. Heim, P. Garrigue, S. Ravaine, N. Mano, A. Kuhn. “Bottom-up Generation of Miniaturized Coaxial Double Electrodes with Tunable Porosity” Adv Mat Interf 2 (2015) 1500192.

P. Kielb, M. Sezer, S. Katz, F. Lopez, C. Schulz, L. Gorton, R. Ludwig, U. Wollenberger, I. Zebger, I.M. Weidinger. „Spectroscopic Observation of Calcium-Induced Reorientation of Cellobiose Dehydrogenase Immobilized on Electrodes and its Effect on Electrocatalytic Activity” ChemPhysChem 16 (2015) 1960-1968.

M. Kizling, S. Draminska, K. Stolarczyk, P. Tammela, Z. Wang, L. Nyholm, R. Bilewicz. “Biosupercapacitors For Powering Oxygen Sensing Devices”, Bioelectrochemistry, 106 (2015), 34-40.

M. Kizling, K. Stolarczyk, J. Sim Sin Kiat, P. Tammela, Z. Wang, L. Nyholm, R. Bilewicz. "Pseudocapacitive polypyrrole–nanocellulose composite for sugar-air enzymatic fuel cells" Electrochem Comm 50 (2015) 55–59.

D. Kracher, K. Zahma, C. Schulz, C. Sygmund, L. Gorton, R. Ludwig. „Inter-domain electron transfer in cellobiose dehydrogenase: modulation by pH and divalent cations” FEBS Journal 282 (2015) 3136-3148 .

D.M. Mate, M. Alcalde. "Laccase engineering: From rational design to directed evolution" Biotechnol Adv 33 (2015) 25-40.

E. Matysiak, A.J.R. Botz, J. Clausmeyer, B. Wagner, W. Schuhmann, Z. Stojek, A.M. Nowicka. "Assembling Paramagnetic Ceruloplasmin at Electrode Surfaces Covered with Ferromagnetic Nanoparticles. Scanning Electrochemical Microscopy in the Presence of a Magnetic Field" Langmuir 31 (2015) 8176-8183.

P. Molina-Espeja, S. Ma, D.M. Mate, R. Ludwig, M. Alcalde. „Tandem-yeast expression system for engineering and producing unspecific peroxygenase” Enzyme and Microbial Technology 73–74 (2015) 29–33.

D. Pankratov, R. Sundberg, J. Sotres, I. Maximov, M. Graczyk, D. Suyatin, E. González-Arribas, A. Lipkin, L. Montelius, S. Shleev. “Transparent and flexible, nanostructured and mediatorless glucose/oxygen enzymatic fuel cells” Journal of Power Sources 294 (2015) 501-506.

P. Pinyou, F. Conzuelo, K. Sliozberg, J. Vivekananthan, A. Contin, S. Pöller, N. Plumeré, W. Schuhmann. "Coupling of an enzymatic biofuel cell to an electrochemical cell for self-powered glucose sensing with optical readout" Bioelectrochemistry 106 (2015) 22-27.

X. Xiao, E. Magner. "A biofuel cell in non-aqueous solution", Chem. Commun. 51 (2015) 13478-13480.

M.E. Yakovleva, C. Gonaus, K. Schropp, P. OConghaile, D. Leech, C.K. Peterbauer, L. Gorton. "Engineering of pyranose dehydrogenase for application to enzymatic anodes in biofuel cells" PCCP 17 (2015) 9074-9081.


X. Chen, M. Shao, S. Pöller, D. A. Guschin, P. Pinyou, W. Schuhmann. "PQQ-sGDH bioelectrodes based on Os-complex modified electrodeposition polymers and carbon nanotubes" J Electrochem Soc 161 (2014) H3058-H3063.

F. Conzuelo, J. Vivekananthan, S. Pöller, J. M. Pingarrón, W. Schuhmann. "Immunologic-controlled biofuel cell as a self-powered biosensor for antibiotic residues determination" ChemElectroChem 1 (2014) 1854-1858.

M. Falk, D. Pankratov, L. Lindh, T. Arnebrant, S. Shleev. "Miniature Direct Electron Transfer Based Enzymatic Fuel Cell Operating in Human Sweat and Saliva" Fuel Cells 14 (6) (2014) 1050-1056.

K. Hasan, Y. Dilgin, S. Emek, M.Tavahodi, H. Akerlund, P. Albertsson, L. Gorton "Photoelectrochemical Communication between Thylakoid Membranes and Gold Electrodes through Different Quinone Derivatives" ChemElectroChem 1 (2014) 131-139.

K. Hasan, H. Yildiz, E. Sperling, P.O´Conghaile, M.A. Packer, D. Leech, C. Hägerhäll, L. Gorton. "Photo-electrochemical communication between cyanobacteria (Leptolyngbia sp.) and osmium redox polymer modified electrodes" PhysChemChemPhys 16 (2014) 24676-24680.

D. Kracher, D. Oros, WY. Yao, M. Preims, I. Rezic, D. Haltrich, T. Rezic, R. Ludwig. „Fungal secretomes enhance sugar beet pulp hydrolysis” Biotech J. 9 (2014) 483-492.

D. Pankratov, R. Sundberg, D. B. Suyatin, J. Sotres, A. Barrantes, T. Ruzgas, I. Maximov, L. Montelius, S.Shleev. "The influence of nanoparticles on enzymatic bioelectrocatalysis" RSC Advances, 4 (2014) 38164.

M. Pita, D. M. Mate, D.Gonzalez-Perez, S. Shleev, V. M. Fernandez, M. Alcalde, A. L. De Lacey. "Bioelectrochemical Oxidation of Water" JACS 136 (2014) 5892−5895.

J. Vivekananthan, R.A. Rincón, V. Kuznetsov, S. Pöller, W. Schuhmann. "Biofuel cell cathodes based on bilirubin oxidase immobilized via organic linkers on 3D-hierarchically structured carbon electrodes" ChemElectroChem 1 (2014) 1901-1908.